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Tumor-specific recognition of human myeloma cells by idiotype-induced CD8(+) T cells.

Publication ,  Journal Article
Li, Y; Bendandi, M; Deng, Y; Dunbar, C; Munshi, N; Jagannath, S; Kwak, LW; Lyerly, HK
Published in: Blood
October 15, 2000

Immunoglobulin secreted by myeloma cells contains a unique antigenic determinant (idiotype [Id]) that may serve as a tumor-specific antigen. Although Id-protein-specific T-cell responses have been reported in patients with myeloma, it is not known whether primary myeloma tumor cells can present naturally processed Id peptides on their surface as a target. We immunized 2 healthy human stem-cell donors with Id proteins from their recipients. T cells from the immunized donors released high levels of T-helper 1-type cytokines in response to stimulation with myeloma cells from their recipients. The T-cell-mediated cytokine response to tumor cells was blocked by a major histocompatibility complex (MHC) class I monoclonal antibody, whereas the response to soluble Id protein was dependent on MHC class II. To investigate whether Id-specific CD8(+) T cells can recognize and kill autologous myeloma cells, we generated T cells from peripheral blood mononuclear cells from a third patient with myeloma by means of in vitro stimulation with autologous dendritic cells pulsed with Id protein. Tumor-specific lysis of myeloma cells was demonstrated by the lack of killing of autologous nonmalignant B cells or natural killer-sensitive K562 cells. Lysis of autologous myeloma targets was restricted by MHC class I molecules. These data represent the first report of class I-restricted T-cell recognition of fresh autologous myeloma targets and formally demonstrate that human myeloma cells can serve as targets of an Id-specific T-cell response. (Blood. 2000;96:2828-2833)

Duke Scholars

Published In

Blood

ISSN

0006-4971

Publication Date

October 15, 2000

Volume

96

Issue

8

Start / End Page

2828 / 2833

Location

United States

Related Subject Headings

  • Myeloma Proteins
  • Multiple Myeloma
  • Middle Aged
  • Male
  • Lymphokines
  • Lymphocyte Activation
  • K562 Cells
  • Immunology
  • Immunoglobulin Idiotypes
  • Immunization
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Li, Y., Bendandi, M., Deng, Y., Dunbar, C., Munshi, N., Jagannath, S., … Lyerly, H. K. (2000). Tumor-specific recognition of human myeloma cells by idiotype-induced CD8(+) T cells. Blood, 96(8), 2828–2833.
Li, Y., M. Bendandi, Y. Deng, C. Dunbar, N. Munshi, S. Jagannath, L. W. Kwak, and H. K. Lyerly. “Tumor-specific recognition of human myeloma cells by idiotype-induced CD8(+) T cells.Blood 96, no. 8 (October 15, 2000): 2828–33.
Li Y, Bendandi M, Deng Y, Dunbar C, Munshi N, Jagannath S, et al. Tumor-specific recognition of human myeloma cells by idiotype-induced CD8(+) T cells. Blood. 2000 Oct 15;96(8):2828–33.
Li, Y., et al. “Tumor-specific recognition of human myeloma cells by idiotype-induced CD8(+) T cells.Blood, vol. 96, no. 8, Oct. 2000, pp. 2828–33.
Li Y, Bendandi M, Deng Y, Dunbar C, Munshi N, Jagannath S, Kwak LW, Lyerly HK. Tumor-specific recognition of human myeloma cells by idiotype-induced CD8(+) T cells. Blood. 2000 Oct 15;96(8):2828–2833.

Published In

Blood

ISSN

0006-4971

Publication Date

October 15, 2000

Volume

96

Issue

8

Start / End Page

2828 / 2833

Location

United States

Related Subject Headings

  • Myeloma Proteins
  • Multiple Myeloma
  • Middle Aged
  • Male
  • Lymphokines
  • Lymphocyte Activation
  • K562 Cells
  • Immunology
  • Immunoglobulin Idiotypes
  • Immunization