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Immunoglobulin-mediated agglutination of and biofilm formation by Escherichia coli K-12 require the type 1 pilus fiber.

Publication ,  Journal Article
Orndorff, PE; Devapali, A; Palestrant, S; Wyse, A; Everett, ML; Bollinger, RR; Parker, W
Published in: Infect Immun
April 2004

The binding of human secretory immunoglobulin A (SIgA), the primary immunoglobulin in the gut, to Escherichia coli is thought to be dependent on type 1 pili. Type 1 pili are filamentous bacterial surface attachment organelles comprised principally of a single protein, the product of the fimA gene. A minor component of the pilus fiber (the product of the fimH gene, termed the adhesin) mediates attachment to a variety of host cell molecules in a mannose inhibitable interaction that has been extensively described. We found that the aggregation of E. coli K-12 by human secretory IgA (SIgA) was dependent on the presence of the pilus fiber, even in the absence of the mannose specific adhesin or in the presence of 25 mM alpha-CH(3)Man. The presence of pilus without adhesin also facilitated SIgA-mediated biofilm formation on polystyrene, although biofilm formation was stronger in the presence of the adhesin. IgM also mediated aggregation and biofilm formation in a manner dependent on pili with or without adhesin. These findings indicate that the pilus fiber, even in the absence of the adhesin, may play a role in biologically important processes. Under conditions in which E. coli was agglutinated by SIgA, the binding of SIgA to E. coli was not increased by the presence of the pili, with or without adhesin. This observation suggests that the pili, with or without adhesin, affect factors such as cell surface rigidity or electrostatic repulsion, which can affect agglutination but which do not necessarily determine the level of bound immunoglobulin.

Duke Scholars

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

April 2004

Volume

72

Issue

4

Start / End Page

1929 / 1938

Location

United States

Related Subject Headings

  • Microbiology
  • Mannose
  • Immunoglobulin A, Secretory
  • Humans
  • Fimbriae, Bacterial
  • Fimbriae Proteins
  • Escherichia coli
  • Biofilms
  • Bacterial Adhesion
  • Agglutination
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Orndorff, P. E., Devapali, A., Palestrant, S., Wyse, A., Everett, M. L., Bollinger, R. R., & Parker, W. (2004). Immunoglobulin-mediated agglutination of and biofilm formation by Escherichia coli K-12 require the type 1 pilus fiber. Infect Immun, 72(4), 1929–1938. https://doi.org/10.1128/IAI.72.4.1929-1938.2004
Orndorff, Paul E., Aditya Devapali, Sarah Palestrant, Aaron Wyse, Mary Lou Everett, R Randal Bollinger, and William Parker. “Immunoglobulin-mediated agglutination of and biofilm formation by Escherichia coli K-12 require the type 1 pilus fiber.Infect Immun 72, no. 4 (April 2004): 1929–38. https://doi.org/10.1128/IAI.72.4.1929-1938.2004.
Orndorff PE, Devapali A, Palestrant S, Wyse A, Everett ML, Bollinger RR, et al. Immunoglobulin-mediated agglutination of and biofilm formation by Escherichia coli K-12 require the type 1 pilus fiber. Infect Immun. 2004 Apr;72(4):1929–38.
Orndorff, Paul E., et al. “Immunoglobulin-mediated agglutination of and biofilm formation by Escherichia coli K-12 require the type 1 pilus fiber.Infect Immun, vol. 72, no. 4, Apr. 2004, pp. 1929–38. Pubmed, doi:10.1128/IAI.72.4.1929-1938.2004.
Orndorff PE, Devapali A, Palestrant S, Wyse A, Everett ML, Bollinger RR, Parker W. Immunoglobulin-mediated agglutination of and biofilm formation by Escherichia coli K-12 require the type 1 pilus fiber. Infect Immun. 2004 Apr;72(4):1929–1938.

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

April 2004

Volume

72

Issue

4

Start / End Page

1929 / 1938

Location

United States

Related Subject Headings

  • Microbiology
  • Mannose
  • Immunoglobulin A, Secretory
  • Humans
  • Fimbriae, Bacterial
  • Fimbriae Proteins
  • Escherichia coli
  • Biofilms
  • Bacterial Adhesion
  • Agglutination