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Antibody-targeted photodynamic therapy.

Publication ,  Journal Article
Mayo, GL; Melendez, RF; Kumar, N; McKinnon, SJ; Glickman, RD
Published in: Am J Ophthalmol
December 2003

PURPOSE: To assess the feasibility of conjugation of verteporfin (Visudyne, Parkedale Pharmaceuticals, Rochester, Minnesota, USA) to antibody against vascular endothelial growth factor. DESIGN: Experimental study. METHODS: Rabbit antimouse vascular endothelial growth factor polyclonal antibody was conjugated to verteporfin. Fluorescence excitation-emission spectra of verteporfin and conjugate were examined. Vascular endothelial growth factor-expressing murine endothelial cells were incubated with saline, verteporfin, or conjugate, followed by laser exposure or no laser exposure. Cell viability at 1 and 24 hours was assessed via trypan blue exclusion. Results were analyzed by two-way analysis of variance with replication and the Bonferroni multiple comparison test. RESULTS: The fluorescence excitation-emission spectrum of the conjugate was similar to that of verteporfin. After laser exposure, cell viability in conjugate-treated cells was reduced to 6% at 1 hour (P <.0001) and to 4% at 24 hours (P <.0001), compared with approximately 40% in nonlaser-exposed, conjugate-treated cells. The cytotoxicity in the conjugate-treated cells was higher than in verteporfin-treated cells exposed to laser, although the difference did not reach statistical significance. CONCLUSIONS: The conjugation of verteporfin to polyclonal antibody is possible without the loss of its photosensitizing properties. Conjugated verteporfin destroys cellular targets at least as effectively as verteporfin alone.

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Published In

Am J Ophthalmol

DOI

ISSN

0002-9394

Publication Date

December 2003

Volume

136

Issue

6

Start / End Page

1151 / 1152

Location

United States

Related Subject Headings

  • Verteporfin
  • Vascular Endothelial Growth Factor A
  • Rabbits
  • Porphyrins
  • Photosensitizing Agents
  • Photochemotherapy
  • Ophthalmology & Optometry
  • Microscopy, Confocal
  • Mice
  • Immunoconjugates
 

Citation

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Mayo, G. L., Melendez, R. F., Kumar, N., McKinnon, S. J., & Glickman, R. D. (2003). Antibody-targeted photodynamic therapy. Am J Ophthalmol, 136(6), 1151–1152. https://doi.org/10.1016/s0002-9394(03)00675-5
Mayo, George L., Robert F. Melendez, Neeru Kumar, Stuart J. McKinnon, and Randolph D. Glickman. “Antibody-targeted photodynamic therapy.Am J Ophthalmol 136, no. 6 (December 2003): 1151–52. https://doi.org/10.1016/s0002-9394(03)00675-5.
Mayo GL, Melendez RF, Kumar N, McKinnon SJ, Glickman RD. Antibody-targeted photodynamic therapy. Am J Ophthalmol. 2003 Dec;136(6):1151–2.
Mayo, George L., et al. “Antibody-targeted photodynamic therapy.Am J Ophthalmol, vol. 136, no. 6, Dec. 2003, pp. 1151–52. Pubmed, doi:10.1016/s0002-9394(03)00675-5.
Mayo GL, Melendez RF, Kumar N, McKinnon SJ, Glickman RD. Antibody-targeted photodynamic therapy. Am J Ophthalmol. 2003 Dec;136(6):1151–1152.
Journal cover image

Published In

Am J Ophthalmol

DOI

ISSN

0002-9394

Publication Date

December 2003

Volume

136

Issue

6

Start / End Page

1151 / 1152

Location

United States

Related Subject Headings

  • Verteporfin
  • Vascular Endothelial Growth Factor A
  • Rabbits
  • Porphyrins
  • Photosensitizing Agents
  • Photochemotherapy
  • Ophthalmology & Optometry
  • Microscopy, Confocal
  • Mice
  • Immunoconjugates