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Retrograde axonal transport of BDNF in retinal ganglion cells is blocked by acute IOP elevation in rats.

Publication ,  Journal Article
Quigley, HA; McKinnon, SJ; Zack, DJ; Pease, ME; Kerrigan-Baumrind, LA; Kerrigan, DF; Mitchell, RS
Published in: Invest Ophthalmol Vis Sci
October 2000

PURPOSE: To determine whether acute experimental glaucoma in rats obstructs retrograde transport of brain-derived neurotrophic factor (BDNF) to retinal ganglion cells (RGCs). METHODS: Forty rats had unilateral injection of either (125)I-BDNF (20 animals) or a mixture of (125)I-BDNF and 100-fold excess nonradiolabeled BDNF (20 animals). In each group of 20 animals, eyes contralateral to injection had either normal intraocular pressure (IOP; 10 animals) or IOP elevated to 25 mm Hg below the systolic blood pressure of the eye (10 animals). In each group of 20 rats, ipsilateral eyes had IOP set at systolic blood pressure (4 eyes), had optic nerve transection (10 eyes), or had normal IOP (6 eyes). Six hours after injection, animals were killed and tissues were fixed, embedded, and sectioned for autoradiography. Grain counts were performed over retina and optic nerve using automated image analysis. RESULTS: IOP elevation to 25 mm Hg below systolic blood pressure (perfusion pressure [PP] 25) decreased median retinal nerve fiber layer (NFL) grains by 38% compared with controls (P: < 0.001). Competition by cold BDNF reduced NFL grains by 28% (P: = 0.013). Considering only the radioactivity representing specific retrograde transport of BDNF, IOP elevation to PP25 reduced transport by 74%, whereas elevation to PP0 (equaling systolic blood pressure) reduced specific transport by 83%. CONCLUSIONS: BDNF is transported retrogradely from the superior colliculus in adult rats, and this transport is substantially inhibited by acute IOP elevation. Deprivation of BDNF among RGCs may contribute to neuron loss in glaucoma.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

October 2000

Volume

41

Issue

11

Start / End Page

3460 / 3466

Location

United States

Related Subject Headings

  • Superior Colliculi
  • Retinal Ganglion Cells
  • Rats, Inbred BN
  • Rats
  • Optic Nerve
  • Optic Disk
  • Ophthalmology & Optometry
  • Ocular Hypertension
  • Nerve Fibers
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Quigley, H. A., McKinnon, S. J., Zack, D. J., Pease, M. E., Kerrigan-Baumrind, L. A., Kerrigan, D. F., & Mitchell, R. S. (2000). Retrograde axonal transport of BDNF in retinal ganglion cells is blocked by acute IOP elevation in rats. Invest Ophthalmol Vis Sci, 41(11), 3460–3466.
Quigley, H. A., S. J. McKinnon, D. J. Zack, M. E. Pease, L. A. Kerrigan-Baumrind, D. F. Kerrigan, and R. S. Mitchell. “Retrograde axonal transport of BDNF in retinal ganglion cells is blocked by acute IOP elevation in rats.Invest Ophthalmol Vis Sci 41, no. 11 (October 2000): 3460–66.
Quigley HA, McKinnon SJ, Zack DJ, Pease ME, Kerrigan-Baumrind LA, Kerrigan DF, et al. Retrograde axonal transport of BDNF in retinal ganglion cells is blocked by acute IOP elevation in rats. Invest Ophthalmol Vis Sci. 2000 Oct;41(11):3460–6.
Quigley, H. A., et al. “Retrograde axonal transport of BDNF in retinal ganglion cells is blocked by acute IOP elevation in rats.Invest Ophthalmol Vis Sci, vol. 41, no. 11, Oct. 2000, pp. 3460–66.
Quigley HA, McKinnon SJ, Zack DJ, Pease ME, Kerrigan-Baumrind LA, Kerrigan DF, Mitchell RS. Retrograde axonal transport of BDNF in retinal ganglion cells is blocked by acute IOP elevation in rats. Invest Ophthalmol Vis Sci. 2000 Oct;41(11):3460–3466.

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

October 2000

Volume

41

Issue

11

Start / End Page

3460 / 3466

Location

United States

Related Subject Headings

  • Superior Colliculi
  • Retinal Ganglion Cells
  • Rats, Inbred BN
  • Rats
  • Optic Nerve
  • Optic Disk
  • Ophthalmology & Optometry
  • Ocular Hypertension
  • Nerve Fibers
  • Male