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Regulation of ribosome detachment from the mammalian endoplasmic reticulum membrane.

Publication ,  Journal Article
Potter, MD; Nicchitta, CV
Published in: J Biol Chem
October 27, 2000

In current models, protein translocation in the endoplasmic reticulum (ER) occurs in the context of two cycles, the signal recognition particle (SRP) cycle and the ribosome cycle. Both SRP and ribosomes bind to the ER membrane as a consequence of the targeting process of translocation. Whereas SRP release from the ER membrane is regulated by the GTPase activities of SRP and the SRP receptor, ribosome release from the ER membrane is thought to occur in response to the termination of protein synthesis. We report that ER-bound ribosomes remain membrane-bound following the termination of protein synthesis and in the bound state can initiate the translation of secretory and cytoplasmic proteins. Two principal observations are reported. 1) Membrane-bound ribosomes engaged in the synthesis of proteins lacking a signal sequence are released from the ER membrane as ribosome-nascent polypeptide complexes. 2) Membrane-bound ribosomes translating secretory proteins can access the translocon in an SRP receptor-independent manner. We propose that ribosome release from the ER membrane occurs in the context of protein translation, with release occurring by default in the absence of productive nascent polypeptide-membrane interactions.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

October 27, 2000

Volume

275

Issue

43

Start / End Page

33828 / 33835

Location

United States

Related Subject Headings

  • Ribosomes
  • Receptors, Peptide
  • Receptors, Cytoplasmic and Nuclear
  • Protein Conformation
  • Protein Biosynthesis
  • Peptides
  • Endoplasmic Reticulum
  • Dogs
  • Cell Membrane
  • Biochemistry & Molecular Biology
 

Citation

APA
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Potter, M. D., & Nicchitta, C. V. (2000). Regulation of ribosome detachment from the mammalian endoplasmic reticulum membrane. J Biol Chem, 275(43), 33828–33835. https://doi.org/10.1074/jbc.M005294200
Potter, M. D., and C. V. Nicchitta. “Regulation of ribosome detachment from the mammalian endoplasmic reticulum membrane.J Biol Chem 275, no. 43 (October 27, 2000): 33828–35. https://doi.org/10.1074/jbc.M005294200.
Potter MD, Nicchitta CV. Regulation of ribosome detachment from the mammalian endoplasmic reticulum membrane. J Biol Chem. 2000 Oct 27;275(43):33828–35.
Potter, M. D., and C. V. Nicchitta. “Regulation of ribosome detachment from the mammalian endoplasmic reticulum membrane.J Biol Chem, vol. 275, no. 43, Oct. 2000, pp. 33828–35. Pubmed, doi:10.1074/jbc.M005294200.
Potter MD, Nicchitta CV. Regulation of ribosome detachment from the mammalian endoplasmic reticulum membrane. J Biol Chem. 2000 Oct 27;275(43):33828–33835.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

October 27, 2000

Volume

275

Issue

43

Start / End Page

33828 / 33835

Location

United States

Related Subject Headings

  • Ribosomes
  • Receptors, Peptide
  • Receptors, Cytoplasmic and Nuclear
  • Protein Conformation
  • Protein Biosynthesis
  • Peptides
  • Endoplasmic Reticulum
  • Dogs
  • Cell Membrane
  • Biochemistry & Molecular Biology