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Mucosal adjuvant effect of cholera toxin in mice results from induction of T helper 2 (Th2) cells and IL-4.

Publication ,  Journal Article
Marinaro, M; Staats, HF; Hiroi, T; Jackson, RJ; Coste, M; Boyaka, PN; Okahashi, N; Yamamoto, M; Kiyono, H; Bluethmann, H; Fujihashi, K; McGhee, JR
Published in: J Immunol
November 15, 1995

Despite pathophysiologic effects including diarrhea, cholera toxin (CT) is a potent mucosal immunogen and adjuvant. We investigated the influence of CT on T helper (Th)-type 1 (Th1) and Th2 cell-regulated Ag-specific B cell isotype and IgG subclass Ab responses elicited when the toxin was co-administered orally with different protein Ags. When mice were orally immunized with tetanus toxoid (TT) and CT as adjuvant, this regimen induced TT-specific secretory IgA responses in the gastrointestinal tract as well as serum IgG, including IgG1 and IgG2b subclasses, and IgA responses. This oral regimen also induced TT- and CT-B-specific IgE responses. In addition, CT also elicited adjuvant effects for Ag-specific IgG1, IgE, and IgA responses when two other protein Ags, OVA and hen egg white lysozyme, were given by the oral route. Quantitative reverse transcriptase-PCR was performed to assess levels of mRNA for Th1 (IFN-gamma) and Th2 (IL-4) cytokine expression in TT-stimulated CD4+ T cell cultures. Both Peyer's patches and splenic CD4+ T cells expressed markedly increased levels of IL-4-specific message, but did not result in changes in IFN-gamma mRNA expression. To determine whether the route of immunization influenced IgE responses, mice were immunized s.c. with TT and CT as adjuvant. Significant increases in total and TT-specific IgE Abs were induced when CT was co-administered. Taken together, these results show that CT acts as a mucosal adjuvant to enhance Th2-type responses and in particular, the IL-4 produced results in a characteristic Ab isotype pattern associated with this cytokine.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

November 15, 1995

Volume

155

Issue

10

Start / End Page

4621 / 4629

Location

United States

Related Subject Headings

  • Th2 Cells
  • Tetanus Toxoid
  • Ovum
  • Muramidase
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Activation
  • Interleukin-4
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Marinaro, M., Staats, H. F., Hiroi, T., Jackson, R. J., Coste, M., Boyaka, P. N., … McGhee, J. R. (1995). Mucosal adjuvant effect of cholera toxin in mice results from induction of T helper 2 (Th2) cells and IL-4. J Immunol, 155(10), 4621–4629.
Marinaro, M., H. F. Staats, T. Hiroi, R. J. Jackson, M. Coste, P. N. Boyaka, N. Okahashi, et al. “Mucosal adjuvant effect of cholera toxin in mice results from induction of T helper 2 (Th2) cells and IL-4.J Immunol 155, no. 10 (November 15, 1995): 4621–29.
Marinaro M, Staats HF, Hiroi T, Jackson RJ, Coste M, Boyaka PN, et al. Mucosal adjuvant effect of cholera toxin in mice results from induction of T helper 2 (Th2) cells and IL-4. J Immunol. 1995 Nov 15;155(10):4621–9.
Marinaro, M., et al. “Mucosal adjuvant effect of cholera toxin in mice results from induction of T helper 2 (Th2) cells and IL-4.J Immunol, vol. 155, no. 10, Nov. 1995, pp. 4621–29.
Marinaro M, Staats HF, Hiroi T, Jackson RJ, Coste M, Boyaka PN, Okahashi N, Yamamoto M, Kiyono H, Bluethmann H, Fujihashi K, McGhee JR. Mucosal adjuvant effect of cholera toxin in mice results from induction of T helper 2 (Th2) cells and IL-4. J Immunol. 1995 Nov 15;155(10):4621–4629.

Published In

J Immunol

ISSN

0022-1767

Publication Date

November 15, 1995

Volume

155

Issue

10

Start / End Page

4621 / 4629

Location

United States

Related Subject Headings

  • Th2 Cells
  • Tetanus Toxoid
  • Ovum
  • Muramidase
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Activation
  • Interleukin-4
  • Immunology