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Modification of the tandem reactive centres of human inter-alpha-trypsin inhibitor with butanedione and cis-dichlorodiammineplatinum(II).

Publication ,  Journal Article
Swaim, MW; Pizzo, SV
Published in: Biochem J
August 15, 1988

Human inter-alpha-trypsin inhibitor (I alpha I) is a plasma proteinase inhibitor active against cathepsin G, leucocyte elastase, trypsin and chymotrypsin. It owes its broad inhibitory specificity to tandem Kunitz-type inhibitory domains within an N-terminal region. Sequence studies suggest that the reactive-centre residues critical for inhibition are methionine and arginine. Reaction of I alpha I with the arginine-modifying reagent butane-2,3-dione afforded partial loss of inhibitory activity against both cathepsin G and elastase but complete loss of activity against trypsin and chymotrypsin. Reaction of I alpha I with the methionine-modifying reagent cis-dichlorodiammineplatinum(II) resulted in partial loss of activity against cathepsin G and elastase but did not affect inhibition of either trypsin or chymotrypsin. Employment of both reagents eliminated inhibition of cathepsin G and elastase. These findings suggest that both cathepsin G and elastase are inhibited at either of the reactive centres of I alpha I. Trypsin and chymotrypsin, however, appear to be inhibited exclusively at the arginine reactive centre.

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Published In

Biochem J

DOI

ISSN

0264-6021

Publication Date

August 15, 1988

Volume

254

Issue

1

Start / End Page

171 / 178

Location

England

Related Subject Headings

  • Trypsin Inhibitors
  • Serine Endopeptidases
  • Pancreatic Elastase
  • Methionine
  • Humans
  • Ethers, Cyclic
  • Epoxy Compounds
  • Cisplatin
  • Chymotrypsin
  • Cathepsins
 

Citation

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Swaim, M. W., & Pizzo, S. V. (1988). Modification of the tandem reactive centres of human inter-alpha-trypsin inhibitor with butanedione and cis-dichlorodiammineplatinum(II). Biochem J, 254(1), 171–178. https://doi.org/10.1042/bj2540171
Swaim, M. W., and S. V. Pizzo. “Modification of the tandem reactive centres of human inter-alpha-trypsin inhibitor with butanedione and cis-dichlorodiammineplatinum(II).Biochem J 254, no. 1 (August 15, 1988): 171–78. https://doi.org/10.1042/bj2540171.
Swaim, M. W., and S. V. Pizzo. “Modification of the tandem reactive centres of human inter-alpha-trypsin inhibitor with butanedione and cis-dichlorodiammineplatinum(II).Biochem J, vol. 254, no. 1, Aug. 1988, pp. 171–78. Pubmed, doi:10.1042/bj2540171.
Journal cover image

Published In

Biochem J

DOI

ISSN

0264-6021

Publication Date

August 15, 1988

Volume

254

Issue

1

Start / End Page

171 / 178

Location

England

Related Subject Headings

  • Trypsin Inhibitors
  • Serine Endopeptidases
  • Pancreatic Elastase
  • Methionine
  • Humans
  • Ethers, Cyclic
  • Epoxy Compounds
  • Cisplatin
  • Chymotrypsin
  • Cathepsins