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The exposure of murine macrophages to alpha 2-macroglobulin 'fast' forms results in the rapid secretion of eicosanoids.

Publication ,  Journal Article
Uhing, RJ; Martenson, CH; Rubenstein, DS; Hollenbach, PW; Pizzo, SV
Published in: Biochim Biophys Acta
July 10, 1991

The exposure of [3H]arachidonate-radiolabelled murine peritoneal macrophages to alpha 2-macroglobulin-methylamine or alpha 2-macroglobulin-trypsin but not native alpha 2-macroglobulin (alpha 2M) results in the rapid secretion of [3H]eicosanoids. Resident peritoneal macrophages stimulated with 0.1 microM alpha 2M-methylamine exhibited an enhanced secretion within 10 min. The ability of alpha 2M 'fast' forms to stimulate secretion of [3H]eicosanoids was similar to that observed in the presence of the murine macrophage chemoattractant platelet-activating factor. As observed for total [3H]eicosanoid secretion, alpha 2M 'fast' forms also rapidly enhanced the secretion of the cAMP-elevating prostanoid, prostaglandin E2, from resident peritoneal macrophages. Stimulated secretion of prostaglandin E2 in response to 0.1 microM alpha 2M-methylamine was less rapid than that observed using 0.1 microM platelet-activating factor. Similar amounts of secreted prostaglandin E2 were present in media of macrophage cultures after 1 h exposure to the two stimuli. In the presence of 0.1 microM alpha 2M-methylamine, secreted prostaglandin E2 remained elevated, compared to the appropriate buffer control, for at least 24 h. The present results indicate that receptor recognition of alpha 2M 'fast' forms by macrophages results in the rapid stimulation of eicosanoid secretion and suggest that secretion of prostaglandin E2 and other eicosanoids may be involved in the ability of alpha 2 M 'fast' forms to regulate various macrophage functional responses.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

July 10, 1991

Volume

1093

Issue

2-3

Start / End Page

115 / 120

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • Trypsin
  • Peritoneum
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Kinetics
  • Humans
  • Eicosanoids
  • Dinoprostone
 

Citation

APA
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ICMJE
MLA
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Uhing, R. J., Martenson, C. H., Rubenstein, D. S., Hollenbach, P. W., & Pizzo, S. V. (1991). The exposure of murine macrophages to alpha 2-macroglobulin 'fast' forms results in the rapid secretion of eicosanoids. Biochim Biophys Acta, 1093(2–3), 115–120. https://doi.org/10.1016/0167-4889(91)90111-a
Uhing, R. J., C. H. Martenson, D. S. Rubenstein, P. W. Hollenbach, and S. V. Pizzo. “The exposure of murine macrophages to alpha 2-macroglobulin 'fast' forms results in the rapid secretion of eicosanoids.Biochim Biophys Acta 1093, no. 2–3 (July 10, 1991): 115–20. https://doi.org/10.1016/0167-4889(91)90111-a.
Uhing RJ, Martenson CH, Rubenstein DS, Hollenbach PW, Pizzo SV. The exposure of murine macrophages to alpha 2-macroglobulin 'fast' forms results in the rapid secretion of eicosanoids. Biochim Biophys Acta. 1991 Jul 10;1093(2–3):115–20.
Uhing, R. J., et al. “The exposure of murine macrophages to alpha 2-macroglobulin 'fast' forms results in the rapid secretion of eicosanoids.Biochim Biophys Acta, vol. 1093, no. 2–3, July 1991, pp. 115–20. Pubmed, doi:10.1016/0167-4889(91)90111-a.
Uhing RJ, Martenson CH, Rubenstein DS, Hollenbach PW, Pizzo SV. The exposure of murine macrophages to alpha 2-macroglobulin 'fast' forms results in the rapid secretion of eicosanoids. Biochim Biophys Acta. 1991 Jul 10;1093(2–3):115–120.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

July 10, 1991

Volume

1093

Issue

2-3

Start / End Page

115 / 120

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • Trypsin
  • Peritoneum
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Kinetics
  • Humans
  • Eicosanoids
  • Dinoprostone