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Selectivity and stereospecificity of the reactions of dichlorodiammineplatinum(II) with three purified plasma proteins.

Publication ,  Journal Article
Pizzo, SV; Swaim, MW; Roche, PA; Gonias, SL
Published in: J Inorg Biochem
May 1988

The reactions of cis- and trans-dichlorodiammineplatinum(II) (cis- and trans-DDP) with albumin and two plasma proteinase inhibitors were compared. Reaction with alpha 2-macroglobulin (alpha 2M) resulted in subunit crosslinking and loss of proteinase binding activity. The reaction also modified a receptor recognition site present on each alpha 2M subunit. While more trans-DDP was incorporated into alpha 2M than cis-DDP, cis-DDP was more effective at blocking receptor recognition, alpha 1-proteinase inhibitor was also inactivated by reaction with either cis- or trans-DDP. These reactions resulted in binding of platinum to methionine-358 at the reactive center of this inhibitor. Trans-DDP, however, was less selective and also bound to the single cysteine residue (Cys-232) of alpha 1PI. Reaction of albumin with cis-DDP resulted in incorporation of about 1 mol platinum per mol protein, and this platinum modified the single cysteine (Cys-34) in the molecule. Albumin incorporated twice as much trans-DDP, but the binding did not involve cysteine-34. In general, reactions of cis-DDP with proteins appear to be more selective than those observed for modification with the trans isomer.

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Published In

J Inorg Biochem

DOI

ISSN

0162-0134

Publication Date

May 1988

Volume

33

Issue

1

Start / End Page

67 / 76

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • alpha 1-Antitrypsin
  • Serum Albumin
  • Protein Binding
  • Protease Inhibitors
  • Molecular Weight
  • Kinetics
  • Isomerism
  • Inorganic & Nuclear Chemistry
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
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Pizzo, S. V., Swaim, M. W., Roche, P. A., & Gonias, S. L. (1988). Selectivity and stereospecificity of the reactions of dichlorodiammineplatinum(II) with three purified plasma proteins. J Inorg Biochem, 33(1), 67–76. https://doi.org/10.1016/0162-0134(88)80035-7
Pizzo, S. V., M. W. Swaim, P. A. Roche, and S. L. Gonias. “Selectivity and stereospecificity of the reactions of dichlorodiammineplatinum(II) with three purified plasma proteins.J Inorg Biochem 33, no. 1 (May 1988): 67–76. https://doi.org/10.1016/0162-0134(88)80035-7.
Pizzo SV, Swaim MW, Roche PA, Gonias SL. Selectivity and stereospecificity of the reactions of dichlorodiammineplatinum(II) with three purified plasma proteins. J Inorg Biochem. 1988 May;33(1):67–76.
Pizzo, S. V., et al. “Selectivity and stereospecificity of the reactions of dichlorodiammineplatinum(II) with three purified plasma proteins.J Inorg Biochem, vol. 33, no. 1, May 1988, pp. 67–76. Pubmed, doi:10.1016/0162-0134(88)80035-7.
Pizzo SV, Swaim MW, Roche PA, Gonias SL. Selectivity and stereospecificity of the reactions of dichlorodiammineplatinum(II) with three purified plasma proteins. J Inorg Biochem. 1988 May;33(1):67–76.
Journal cover image

Published In

J Inorg Biochem

DOI

ISSN

0162-0134

Publication Date

May 1988

Volume

33

Issue

1

Start / End Page

67 / 76

Location

United States

Related Subject Headings

  • alpha-Macroglobulins
  • alpha 1-Antitrypsin
  • Serum Albumin
  • Protein Binding
  • Protease Inhibitors
  • Molecular Weight
  • Kinetics
  • Isomerism
  • Inorganic & Nuclear Chemistry
  • Humans