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Altered clearance of human alpha 2-macroglobulin complexes following reaction with cis-dichlorodiamineplatinum(II).

Publication ,  Journal Article
Gonias, SL; Pizzo, SV
Published in: Biochim Biophys Acta
December 4, 1981

Clearance studies were performed in mice using alpha 2-macroglobulin (alpha 2M), alpha 2M-trypsin complex and alpha 2M-CH3NH2 complex. All three species were incubated with cis-dichlorodiamine platinum(II) (cis-DDPt) at concentrations between 9.0 microM and 1.67 mM for 4 h and then dialyzed. The clearance rate of native alpha 2M was unchanged following incubation with cis-DDPt. alpha 2M-trypsin and alpha 2M-CH3NH2 cleared rapidly from the circulation; however, reaction with cis-DDPt significantly decreased the plasma elimination rate of both complexes. Non-denaturing gel electrophoresis and alpha 2M activity assays demonstrated relative stability following incubations with cis-DDPt which markedly altered clearance. Evidence for cis-DDPt crosslinking of alpha 2M subunits was obtained; however, whether this crosslinking is involved in altered clearance remains undetermined. Iodoacetamide treatment of alpha 2M did not duplicate the effect of cis-DDPt on alpha 2M clearance, nor did it inhibit the effect of cis-DPPt on alpha 2M clearance. Plasma elimination of alpha 2M complex was also unaltered by pretreatment of mice with intravenous free cis-DDPt.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

December 4, 1981

Volume

678

Issue

2

Start / End Page

268 / 274

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • Trypsin
  • Mice
  • Kinetics
  • Iodoacetamide
  • Humans
  • Cisplatin
  • Animals
  • 51 Physical sciences
  • 31 Biological sciences
 

Citation

APA
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ICMJE
MLA
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Gonias, S. L., & Pizzo, S. V. (1981). Altered clearance of human alpha 2-macroglobulin complexes following reaction with cis-dichlorodiamineplatinum(II). Biochim Biophys Acta, 678(2), 268–274. https://doi.org/10.1016/0304-4165(81)90216-6
Gonias, S. L., and S. V. Pizzo. “Altered clearance of human alpha 2-macroglobulin complexes following reaction with cis-dichlorodiamineplatinum(II).Biochim Biophys Acta 678, no. 2 (December 4, 1981): 268–74. https://doi.org/10.1016/0304-4165(81)90216-6.
Gonias, S. L., and S. V. Pizzo. “Altered clearance of human alpha 2-macroglobulin complexes following reaction with cis-dichlorodiamineplatinum(II).Biochim Biophys Acta, vol. 678, no. 2, Dec. 1981, pp. 268–74. Pubmed, doi:10.1016/0304-4165(81)90216-6.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

December 4, 1981

Volume

678

Issue

2

Start / End Page

268 / 274

Location

Netherlands

Related Subject Headings

  • alpha-Macroglobulins
  • Trypsin
  • Mice
  • Kinetics
  • Iodoacetamide
  • Humans
  • Cisplatin
  • Animals
  • 51 Physical sciences
  • 31 Biological sciences