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Orientation and length of mammalian skeletal myocytes in response to a unidirectional stretch.

Publication ,  Journal Article
Collinsworth, AM; Torgan, CE; Nagda, SN; Rajalingam, RJ; Kraus, WE; Truskey, GA
Published in: Cell Tissue Res
November 2000

Effects of mechanical forces exerted on mammalian skeletal muscle cells during development were studied using an in vitro model to unidirectionally stretch cultured C2C12 cells grown on silastic membrane. Previous models to date have not studied these responses of the mammalian system specifically. The silastic membrane upon which these cells were grown exhibited linear strain behavior over the range of 3.6-14.6% strain, with a Poisson's ratio of approximately 0.5. To mimic murine in utero long bone growth, cell substrates were stretched at an average strain rate of 2.36%/day for 4 days or 1.77%/day for 6 days with an overall membrane strain of 9.5% and 10.6%, respectively. Both control and stretched fibers stained positively for the contractile protein, alpha-actinin, demonstrating muscle fiber development. An effect of stretch on orientation and length of myofibers was observed. At both strain rates, stretched fibers aligned at a smaller angle relative to the direction of stretch and were significantly longer compared to randomly oriented control fibers. There was no effect of duration of stretch on orientation or length, suggesting the cellular responses are independent of strain rate for the range tested. These results demonstrate that, under conditions simulating mammalian long bone growth, cultured myocytes respond to mechanical forces by lengthening and orienting along the direction of stretch.

Duke Scholars

Published In

Cell Tissue Res

DOI

ISSN

0302-766X

Publication Date

November 2000

Volume

302

Issue

2

Start / End Page

243 / 251

Location

Germany

Related Subject Headings

  • Stress, Mechanical
  • Neurology & Neurosurgery
  • Muscle, Skeletal
  • Muscle Fibers, Skeletal
  • Mice
  • Cell Line
  • Animals
  • 3208 Medical physiology
  • 1116 Medical Physiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Collinsworth, A. M., Torgan, C. E., Nagda, S. N., Rajalingam, R. J., Kraus, W. E., & Truskey, G. A. (2000). Orientation and length of mammalian skeletal myocytes in response to a unidirectional stretch. Cell Tissue Res, 302(2), 243–251. https://doi.org/10.1007/s004410000224
Collinsworth, A. M., C. E. Torgan, S. N. Nagda, R. J. Rajalingam, W. E. Kraus, and G. A. Truskey. “Orientation and length of mammalian skeletal myocytes in response to a unidirectional stretch.Cell Tissue Res 302, no. 2 (November 2000): 243–51. https://doi.org/10.1007/s004410000224.
Collinsworth AM, Torgan CE, Nagda SN, Rajalingam RJ, Kraus WE, Truskey GA. Orientation and length of mammalian skeletal myocytes in response to a unidirectional stretch. Cell Tissue Res. 2000 Nov;302(2):243–51.
Collinsworth, A. M., et al. “Orientation and length of mammalian skeletal myocytes in response to a unidirectional stretch.Cell Tissue Res, vol. 302, no. 2, Nov. 2000, pp. 243–51. Pubmed, doi:10.1007/s004410000224.
Collinsworth AM, Torgan CE, Nagda SN, Rajalingam RJ, Kraus WE, Truskey GA. Orientation and length of mammalian skeletal myocytes in response to a unidirectional stretch. Cell Tissue Res. 2000 Nov;302(2):243–251.
Journal cover image

Published In

Cell Tissue Res

DOI

ISSN

0302-766X

Publication Date

November 2000

Volume

302

Issue

2

Start / End Page

243 / 251

Location

Germany

Related Subject Headings

  • Stress, Mechanical
  • Neurology & Neurosurgery
  • Muscle, Skeletal
  • Muscle Fibers, Skeletal
  • Mice
  • Cell Line
  • Animals
  • 3208 Medical physiology
  • 1116 Medical Physiology