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Pharmacology of pertussis toxin B-oligomer.

Publication ,  Journal Article
Wong, WS; Rosoff, PM
Published in: Can J Physiol Pharmacol
May 1996

Pertussis toxin (PTX) is a heterohexameric protein, which is divided into subunits A and B. The A-subunit (protomer) possesses adenine diphosphate (ADP) ribosyltransferase activity, and the B-oligomer confers cell surface binding specificity on the toxin. By virtue of the ADP-ribosylation activity in the A-subunit, PTX has become a very useful pharmacological tool for the identification of inhibitory guanine nucleotide binding (Gi) proteins in the plasma membrane. However, the pharmacological properties of the PTX B-oligomer are largely unknown. In the course of identifying its binding site(s), PTX B-oligomer was recently found to elicit direct cellular responses in a variety of cell types. Several cell surface receptors with oligosaccharide side chains have been shown to be specifically bound by PTX B-oligomer. Moreover, occupation of these putative receptors by the B-oligomer alone can trigger phospholipase C and tyrosine kinase dependent signal transduction events. The impact of these B-oligomer-mediated rapid signaling responses on the subsequent ADP-ribosylation of Gi protein by the A-subunit remains to be determined. These recent findings caution investigators not to attribute inhibitory effects of PTX solely to ADP-ribosylation of Gi protein without first examining the cellular responses using PTX B-oligomer.

Duke Scholars

Published In

Can J Physiol Pharmacol

DOI

ISSN

0008-4212

Publication Date

May 1996

Volume

74

Issue

5

Start / End Page

559 / 564

Location

Canada

Related Subject Headings

  • Whooping Cough
  • Virulence Factors, Bordetella
  • Type C Phospholipases
  • Signal Transduction
  • Protein Kinase C
  • Protein Binding
  • Physiology
  • Pertussis Toxin
  • Oligosaccharides
  • Humans
 

Citation

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ICMJE
MLA
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Wong, W. S., & Rosoff, P. M. (1996). Pharmacology of pertussis toxin B-oligomer. Can J Physiol Pharmacol, 74(5), 559–564. https://doi.org/10.1139/cjpp-74-5-559
Wong, W. S., and P. M. Rosoff. “Pharmacology of pertussis toxin B-oligomer.Can J Physiol Pharmacol 74, no. 5 (May 1996): 559–64. https://doi.org/10.1139/cjpp-74-5-559.
Wong WS, Rosoff PM. Pharmacology of pertussis toxin B-oligomer. Can J Physiol Pharmacol. 1996 May;74(5):559–64.
Wong, W. S., and P. M. Rosoff. “Pharmacology of pertussis toxin B-oligomer.Can J Physiol Pharmacol, vol. 74, no. 5, May 1996, pp. 559–64. Pubmed, doi:10.1139/cjpp-74-5-559.
Wong WS, Rosoff PM. Pharmacology of pertussis toxin B-oligomer. Can J Physiol Pharmacol. 1996 May;74(5):559–564.

Published In

Can J Physiol Pharmacol

DOI

ISSN

0008-4212

Publication Date

May 1996

Volume

74

Issue

5

Start / End Page

559 / 564

Location

Canada

Related Subject Headings

  • Whooping Cough
  • Virulence Factors, Bordetella
  • Type C Phospholipases
  • Signal Transduction
  • Protein Kinase C
  • Protein Binding
  • Physiology
  • Pertussis Toxin
  • Oligosaccharides
  • Humans