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4,4'-Diisothiocyanatostilbene-2,2'-disulfonic acid inhibits CD3-T cell antigen receptor-stimulated Ca2+ influx in human T lymphocytes.

Publication ,  Journal Article
Rosoff, PM; Hall, C; Gramates, LS; Terlecky, SR
Published in: J Biol Chem
December 25, 1988

Stimulation of the CD3-T cell antigen receptor complex on T lymphocytes results in a rapid rise in intracellular calcium from both intra- and extracellular sources. The former is thought to be released from the endoplasmic reticulum in response to inositol trisphosphate, while the latter enters the cells through a membrane potential-sensitive transporter (Oettgen, H. C., Terhorst, C., Cantley, L. C., and Rosoff, P. M. (1985) Cell 40, 583-590). In this report we show that the stilbene disulfonate, DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid), inhibited the ability of monoclonal anti-CD3 complex antibodies to stimulate an influx of calcium in the human T lymphocyte cell line, Jurkat. DIDS had no effect on either antibody binding to the receptor or receptor-stimulated phosphatidylinositol turnover. The Ki was approximately 25 microM in the presence of extracellular Cl- and 10 microM when labeling was performed in the absence of Cl-, suggesting that DIDS was competing with Cl- for binding to the cell membrane. The reduced form of DIDS, dihydroDIDS, was only 50% as effective as DIDS itself, and the monoisothiocyanate stilbene, 4-acetamido-4'-isothiocyantostilbene-2,2'-disulfonic acid, was totally ineffective, even to concentrations of 0.750 mM. Removal of extracellular Cl- also inhibited the antibody-stimulated influx of calcium. These data suggest that the function of the CD3-T cell receptor-activated calcium channel/transporter may be dependent on or regulated by extracellular Cl-.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

December 25, 1988

Volume

263

Issue

36

Start / End Page

19535 / 19540

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Stilbenes
  • Receptors, Antigen, T-Cell
  • Kinetics
  • Humans
  • Egtazic Acid
  • Cell Line
  • Calcium
  • CD3 Complex
  • Biological Transport
 

Citation

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Rosoff, P. M., Hall, C., Gramates, L. S., & Terlecky, S. R. (1988). 4,4'-Diisothiocyanatostilbene-2,2'-disulfonic acid inhibits CD3-T cell antigen receptor-stimulated Ca2+ influx in human T lymphocytes. J Biol Chem, 263(36), 19535–19540.
Rosoff, P. M., C. Hall, L. S. Gramates, and S. R. Terlecky. “4,4'-Diisothiocyanatostilbene-2,2'-disulfonic acid inhibits CD3-T cell antigen receptor-stimulated Ca2+ influx in human T lymphocytes.J Biol Chem 263, no. 36 (December 25, 1988): 19535–40.
Rosoff PM, Hall C, Gramates LS, Terlecky SR. 4,4'-Diisothiocyanatostilbene-2,2'-disulfonic acid inhibits CD3-T cell antigen receptor-stimulated Ca2+ influx in human T lymphocytes. J Biol Chem. 1988 Dec 25;263(36):19535–40.
Rosoff PM, Hall C, Gramates LS, Terlecky SR. 4,4'-Diisothiocyanatostilbene-2,2'-disulfonic acid inhibits CD3-T cell antigen receptor-stimulated Ca2+ influx in human T lymphocytes. J Biol Chem. 1988 Dec 25;263(36):19535–19540.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

December 25, 1988

Volume

263

Issue

36

Start / End Page

19535 / 19540

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Stilbenes
  • Receptors, Antigen, T-Cell
  • Kinetics
  • Humans
  • Egtazic Acid
  • Cell Line
  • Calcium
  • CD3 Complex
  • Biological Transport