Skip to main content
Journal cover image

Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line.

Publication ,  Journal Article
Rosoff, PM; Cantley, LC
Published in: Proc Natl Acad Sci U S A
December 1983

The ability of lipopolysaccharide (LPS), a polyclonal B-cell mitogen, to induce differentiation in the pre-B cell tumor line 70Z/3 can be mimicked by drugs that increase the intracellular Na+ concentration. Pharmacologically increasing the cellular Na+ content with monensin, a sodium ionophore, or ouabain, a specific inhibitor of Na+, K+-ATPase, induces surface IgM expression in these cells. We have shown that LPS stimulates uptake of Na+ through an amiloride-sensitive pathway. These results show that the essential action of LPS to induce surface IgM expression is activation of a Na+ uptake system.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 1983

Volume

80

Issue

24

Start / End Page

7547 / 7550

Location

United States

Related Subject Headings

  • Splenic Neoplasms
  • Sodium
  • Monensin
  • Mice
  • Lipopolysaccharides
  • Kinetics
  • Cell Line
  • Cell Differentiation
  • B-Lymphocytes
  • Antibody Formation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Rosoff, P. M., & Cantley, L. C. (1983). Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line. Proc Natl Acad Sci U S A, 80(24), 7547–7550. https://doi.org/10.1073/pnas.80.24.7547
Rosoff, P. M., and L. C. Cantley. “Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line.Proc Natl Acad Sci U S A 80, no. 24 (December 1983): 7547–50. https://doi.org/10.1073/pnas.80.24.7547.
Rosoff PM, Cantley LC. Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7547–50.
Rosoff, P. M., and L. C. Cantley. “Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line.Proc Natl Acad Sci U S A, vol. 80, no. 24, Dec. 1983, pp. 7547–50. Pubmed, doi:10.1073/pnas.80.24.7547.
Rosoff PM, Cantley LC. Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7547–7550.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 1983

Volume

80

Issue

24

Start / End Page

7547 / 7550

Location

United States

Related Subject Headings

  • Splenic Neoplasms
  • Sodium
  • Monensin
  • Mice
  • Lipopolysaccharides
  • Kinetics
  • Cell Line
  • Cell Differentiation
  • B-Lymphocytes
  • Antibody Formation