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Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants.

Publication ,  Journal Article
Traver, D; Paw, BH; Poss, KD; Penberthy, WT; Lin, S; Zon, LI
Published in: Nat Immunol
December 2003

The zebrafish is firmly established as a genetic model for the study of vertebrate blood development. Here we have characterized the blood-forming system of adult zebrafish. Each major blood lineage can be isolated by flow cytometry, and with these lineal profiles, defects in zebrafish blood mutants can be quantified. We developed hematopoietic cell transplantation to study cell autonomy of mutant gene function and to establish a hematopoietic stem cell assay. Hematopoietic cell transplantation can rescue multilineage hematopoiesis in embryonic lethal gata1-/- mutants for over 6 months. Direct visualization of fluorescent donor cells in embryonic recipients allows engraftment and homing events to be imaged in real time. These results provide a cellular context in which to study the genetics of hematopoiesis.

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Published In

Nat Immunol

DOI

ISSN

1529-2908

Publication Date

December 2003

Volume

4

Issue

12

Start / End Page

1238 / 1246

Location

United States

Related Subject Headings

  • Zebrafish
  • Spleen
  • Mutation
  • Kidney
  • Immunology
  • Hematopoietic System
  • Hematopoietic Stem Cells
  • Hematopoietic Stem Cell Transplantation
  • Flow Cytometry
  • Cell Lineage
 

Citation

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Traver, D., Paw, B. H., Poss, K. D., Penberthy, W. T., Lin, S., & Zon, L. I. (2003). Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants. Nat Immunol, 4(12), 1238–1246. https://doi.org/10.1038/ni1007
Traver, David, Barry H. Paw, Kenneth D. Poss, W Todd Penberthy, Shuo Lin, and Leonard I. Zon. “Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants.Nat Immunol 4, no. 12 (December 2003): 1238–46. https://doi.org/10.1038/ni1007.
Traver D, Paw BH, Poss KD, Penberthy WT, Lin S, Zon LI. Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants. Nat Immunol. 2003 Dec;4(12):1238–46.
Traver, David, et al. “Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants.Nat Immunol, vol. 4, no. 12, Dec. 2003, pp. 1238–46. Pubmed, doi:10.1038/ni1007.
Traver D, Paw BH, Poss KD, Penberthy WT, Lin S, Zon LI. Transplantation and in vivo imaging of multilineage engraftment in zebrafish bloodless mutants. Nat Immunol. 2003 Dec;4(12):1238–1246.

Published In

Nat Immunol

DOI

ISSN

1529-2908

Publication Date

December 2003

Volume

4

Issue

12

Start / End Page

1238 / 1246

Location

United States

Related Subject Headings

  • Zebrafish
  • Spleen
  • Mutation
  • Kidney
  • Immunology
  • Hematopoietic System
  • Hematopoietic Stem Cells
  • Hematopoietic Stem Cell Transplantation
  • Flow Cytometry
  • Cell Lineage