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Targeted gene deletion of heme oxygenase 2 reveals neural role for carbon monoxide.

Publication ,  Journal Article
Zakhary, R; Poss, KD; Jaffrey, SR; Ferris, CD; Tonegawa, S; Snyder, SH
Published in: Proc Natl Acad Sci U S A
December 23, 1997

Neuronal nitric oxide synthase (nNOS) generates NO in neurons, and heme-oxygenase-2 (HO-2) synthesizes carbon monoxide (CO). We have evaluated the roles of NO and CO in intestinal neurotransmission using mice with targeted deletions of nNOS or HO-2. Immunohistochemical analysis demonstrated colocalization of nNOS and HO-2 in myenteric ganglia. Nonadrenergic noncholinergic relaxation and cyclic guanosine 3',5' monophosphate elevations evoked by electrical field stimulation were diminished markedly in both nNOSDelta/Delta and HO-2(Delta)/Delta mice. In wild-type mice, NOS inhibitors and HO inhibitors partially inhibited nonadrenergic noncholinergic relaxation. In nNOSDelta/Delta animals, NOS inhibitors selectively lost their efficacy, and HO inhibitors were inactive in HO-2(Delta)/Delta animals.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 23, 1997

Volume

94

Issue

26

Start / End Page

14848 / 14853

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Nitric Oxide Synthase
  • Nitric Oxide
  • Mice
  • Male
  • Heme Oxygenase (Decyclizing)
  • Gene Targeting
  • Gene Deletion
  • Enteric Nervous System
 

Citation

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Zakhary, R., Poss, K. D., Jaffrey, S. R., Ferris, C. D., Tonegawa, S., & Snyder, S. H. (1997). Targeted gene deletion of heme oxygenase 2 reveals neural role for carbon monoxide. Proc Natl Acad Sci U S A, 94(26), 14848–14853. https://doi.org/10.1073/pnas.94.26.14848
Zakhary, R., K. D. Poss, S. R. Jaffrey, C. D. Ferris, S. Tonegawa, and S. H. Snyder. “Targeted gene deletion of heme oxygenase 2 reveals neural role for carbon monoxide.Proc Natl Acad Sci U S A 94, no. 26 (December 23, 1997): 14848–53. https://doi.org/10.1073/pnas.94.26.14848.
Zakhary R, Poss KD, Jaffrey SR, Ferris CD, Tonegawa S, Snyder SH. Targeted gene deletion of heme oxygenase 2 reveals neural role for carbon monoxide. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14848–53.
Zakhary, R., et al. “Targeted gene deletion of heme oxygenase 2 reveals neural role for carbon monoxide.Proc Natl Acad Sci U S A, vol. 94, no. 26, Dec. 1997, pp. 14848–53. Pubmed, doi:10.1073/pnas.94.26.14848.
Zakhary R, Poss KD, Jaffrey SR, Ferris CD, Tonegawa S, Snyder SH. Targeted gene deletion of heme oxygenase 2 reveals neural role for carbon monoxide. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14848–14853.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 23, 1997

Volume

94

Issue

26

Start / End Page

14848 / 14853

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Nitric Oxide Synthase
  • Nitric Oxide
  • Mice
  • Male
  • Heme Oxygenase (Decyclizing)
  • Gene Targeting
  • Gene Deletion
  • Enteric Nervous System