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Roles for Fgf signaling during zebrafish fin regeneration.

Publication ,  Journal Article
Poss, KD; Shen, J; Nechiporuk, A; McMahon, G; Thisse, B; Thisse, C; Keating, MT
Published in: Dev Biol
June 15, 2000

Following amputation of a urodele limb or teleost fin, the formation of a blastema is a crucial step in facilitating subsequent regeneration. Using the zebrafish caudal fin regeneration model, we have examined the hypothesis that fibroblast growth factors (Fgfs) initiate blastema formation from fin mesenchyme. We find that fibroblast growth factor receptor 1 (fgfr1) is expressed in mesenchymal cells underlying the wound epidermis during blastema formation and in distal blastemal tissue during regenerative outgrowth. fgfr1 transcripts colocalize with those of msxb and msxc, putative markers for undifferentiated, proliferating cells. A zebrafish Fgf member, designated wfgf, is expressed in the regeneration epidermis during outgrowth. Furthermore, we show that a specific inhibitor of Fgfr1 applied immediately following fin amputation blocks blastema formation, without obvious effects on wound healing. This inhibitor blocks the proliferation of blastemal cells and the onset of msx gene transcription. Inhibition of Fgf signaling during ongoing fin regeneration prevents further outgrowth while downregulating the established expression of blastemal msx genes and epidermal sonic hedgehog. Our findings indicate that zebrafish fin blastema formation and regenerative outgrowth require Fgf signaling.

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Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

June 15, 2000

Volume

222

Issue

2

Start / End Page

347 / 358

Location

United States

Related Subject Headings

  • Zebrafish
  • Signal Transduction
  • Regeneration
  • Receptors, Fibroblast Growth Factor
  • Receptor, Fibroblast Growth Factor, Type 1
  • Receptor Protein-Tyrosine Kinases
  • Pyrroles
  • Mesoderm
  • Fibroblast Growth Factors
  • Extremities
 

Citation

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Poss, K. D., Shen, J., Nechiporuk, A., McMahon, G., Thisse, B., Thisse, C., & Keating, M. T. (2000). Roles for Fgf signaling during zebrafish fin regeneration. Dev Biol, 222(2), 347–358. https://doi.org/10.1006/dbio.2000.9722
Poss, K. D., J. Shen, A. Nechiporuk, G. McMahon, B. Thisse, C. Thisse, and M. T. Keating. “Roles for Fgf signaling during zebrafish fin regeneration.Dev Biol 222, no. 2 (June 15, 2000): 347–58. https://doi.org/10.1006/dbio.2000.9722.
Poss KD, Shen J, Nechiporuk A, McMahon G, Thisse B, Thisse C, et al. Roles for Fgf signaling during zebrafish fin regeneration. Dev Biol. 2000 Jun 15;222(2):347–58.
Poss, K. D., et al. “Roles for Fgf signaling during zebrafish fin regeneration.Dev Biol, vol. 222, no. 2, June 2000, pp. 347–58. Pubmed, doi:10.1006/dbio.2000.9722.
Poss KD, Shen J, Nechiporuk A, McMahon G, Thisse B, Thisse C, Keating MT. Roles for Fgf signaling during zebrafish fin regeneration. Dev Biol. 2000 Jun 15;222(2):347–358.
Journal cover image

Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

June 15, 2000

Volume

222

Issue

2

Start / End Page

347 / 358

Location

United States

Related Subject Headings

  • Zebrafish
  • Signal Transduction
  • Regeneration
  • Receptors, Fibroblast Growth Factor
  • Receptor, Fibroblast Growth Factor, Type 1
  • Receptor Protein-Tyrosine Kinases
  • Pyrroles
  • Mesoderm
  • Fibroblast Growth Factors
  • Extremities