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Positional cloning of a temperature-sensitive mutant emmental reveals a role for sly1 during cell proliferation in zebrafish fin regeneration.

Publication ,  Journal Article
Nechiporuk, A; Poss, KD; Johnson, SL; Keating, MT
Published in: Dev Biol
June 15, 2003

Here, we used classical genetics in zebrafish to identify temperature-sensitive mutants in caudal fin regeneration. Gross morphological, histological, and molecular analyses revealed that one of these strains, emmental (emm), failed to form a functional regeneration blastema. Inhibition of emm function by heat treatment during regenerative outgrowth rapidly blocked regeneration. This block was associated with reduced proliferation in the proximal blastema and expansion of the nonproliferative distal blastemal zone. Positional cloning revealed that the emm phenotype is caused by a mutation in the orthologue of yeast sly1, a gene product involved in protein trafficking. sly1 is upregulated in the newly formed blastema as well as during regenerative outgrowth. Thus, sly1 is essential for blastemal organization and proliferation during two stages of fin regeneration.

Duke Scholars

Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

June 15, 2003

Volume

258

Issue

2

Start / End Page

291 / 306

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Temperature
  • Sequence Homology, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Regeneration
  • Mutation
  • Molecular Sequence Data
  • Male
  • Laminin
 

Citation

APA
Chicago
ICMJE
MLA
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Nechiporuk, A., Poss, K. D., Johnson, S. L., & Keating, M. T. (2003). Positional cloning of a temperature-sensitive mutant emmental reveals a role for sly1 during cell proliferation in zebrafish fin regeneration. Dev Biol, 258(2), 291–306. https://doi.org/10.1016/s0012-1606(03)00129-5
Nechiporuk, Alex, Kenneth D. Poss, Stephen L. Johnson, and Mark T. Keating. “Positional cloning of a temperature-sensitive mutant emmental reveals a role for sly1 during cell proliferation in zebrafish fin regeneration.Dev Biol 258, no. 2 (June 15, 2003): 291–306. https://doi.org/10.1016/s0012-1606(03)00129-5.
Nechiporuk, Alex, et al. “Positional cloning of a temperature-sensitive mutant emmental reveals a role for sly1 during cell proliferation in zebrafish fin regeneration.Dev Biol, vol. 258, no. 2, June 2003, pp. 291–306. Pubmed, doi:10.1016/s0012-1606(03)00129-5.
Journal cover image

Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

June 15, 2003

Volume

258

Issue

2

Start / End Page

291 / 306

Location

United States

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Temperature
  • Sequence Homology, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Regeneration
  • Mutation
  • Molecular Sequence Data
  • Male
  • Laminin