Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Expression of Sry, the mouse sex determining gene.

Publication ,  Journal Article
Hacker, A; Capel, B; Goodfellow, P; Lovell-Badge, R
Published in: Development
June 1995

In the mouse, Sry is expressed by germ cells in the adult testis and by somatic cells in the genital ridge. Transcripts in the former exist as circular RNA molecules of 1.23 kb, which are unlikely to be efficiently translated. We have used RNase protection to map the extent of the less abundant Sry transcript in the developing gonad. We demonstrate that it is a linear mRNA derived from a single exon. This begins in the unique region 5' of the protein coding region and extends several kilobases into the 3' arm of the large inverted repeat which bounds the Sry genomic locus. Knowledge of this transcript, which is very different from that of the human SRY gene, allows us to predict its protein product and reveals several features which may be involved in translational control. Our data is also consistent with there being two promoters for the Sry gene, a proximal one that gives functional transcripts in the genital ridge and a distal promoter used in germ cells in the adult testis. As RNase protection is a quantitative technique, a detailed timecourse of Sry expression was carried out using accurately staged samples. Sry transcripts are first detectable just after 10.5 days post coitum, they reach a peak at 11.5 days and then decline sharply so that none are detected 24 hours later. This was compared with anti-Müllerian hormone gene expression, an early marker of Sertoli cells and the first known downstream gene of Sry. Amh expression begins 20 hours after the onset of Sry expression at a time when Sry transcripts are at their peak. While this result does not prove a direct interaction between the two genes, it defines the critical period during which Sry must act to initiate Sertoli cell differentiation.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Development

DOI

ISSN

0950-1991

Publication Date

June 1995

Volume

121

Issue

6

Start / End Page

1603 / 1614

Location

England

Related Subject Headings

  • Transcription Factors
  • Testis
  • Spermatozoa
  • Sex-Determining Region Y Protein
  • Sex Determination Analysis
  • Sequence Homology
  • Nuclear Proteins
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hacker, A., Capel, B., Goodfellow, P., & Lovell-Badge, R. (1995). Expression of Sry, the mouse sex determining gene. Development, 121(6), 1603–1614. https://doi.org/10.1242/dev.121.6.1603
Hacker, A., B. Capel, P. Goodfellow, and R. Lovell-Badge. “Expression of Sry, the mouse sex determining gene.Development 121, no. 6 (June 1995): 1603–14. https://doi.org/10.1242/dev.121.6.1603.
Hacker A, Capel B, Goodfellow P, Lovell-Badge R. Expression of Sry, the mouse sex determining gene. Development. 1995 Jun;121(6):1603–14.
Hacker, A., et al. “Expression of Sry, the mouse sex determining gene.Development, vol. 121, no. 6, June 1995, pp. 1603–14. Pubmed, doi:10.1242/dev.121.6.1603.
Hacker A, Capel B, Goodfellow P, Lovell-Badge R. Expression of Sry, the mouse sex determining gene. Development. 1995 Jun;121(6):1603–1614.
Journal cover image

Published In

Development

DOI

ISSN

0950-1991

Publication Date

June 1995

Volume

121

Issue

6

Start / End Page

1603 / 1614

Location

England

Related Subject Headings

  • Transcription Factors
  • Testis
  • Spermatozoa
  • Sex-Determining Region Y Protein
  • Sex Determination Analysis
  • Sequence Homology
  • Nuclear Proteins
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice