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Establishment and characterization of conditionally immortalized cells from the mouse urogenital ridge.

Publication ,  Journal Article
Capel, B; Hawkins, JR; Hirst, E; Kioussis, D; Lovell Badge, R
Published in: Journal of cell science
May 1996

Cell cultures from the urogenital ridge have been established to facilitate the study of the regulation and downstream interactions of Sry in mammalian sex determination. Cells have been explanted from transgenic mice carrying a temperature sensitive SV40 large T-antigen, and established in ongoing cultures. Analysis of the cells in these cultures at the electron microscope level reveals multiple cell types that compare to the cell types found in vivo during this period of development. Primordial germ cells, that are simultaneously explanted in the course of these experiments, also survive in culture. The explants undergo a morphogenetic organization into branching cord-like structures when cells are trypsinized and plated in extracellular matrix (Matrigel). We analyzed the expression of a number of molecular markers of the fetal gonad during monolayer culture, during in vitro morphogenesis in Matrigel, and in clonal lines derived from the complex explants. This analysis included Sry which is found to be expressed in some cultures from XY urogenital ridges that have been maintained for as long as 8 months.

Duke Scholars

Published In

Journal of cell science

ISSN

0021-9533

Publication Date

May 1996

Volume

109 ( Pt 5)

Start / End Page

899 / 909

Location

england

Related Subject Headings

  • Urogenital System
  • Temperature
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Male
  • Germ Cells
  • Female
  • Extracellular Matrix
  • Developmental Biology
 

Citation

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Capel, B., Hawkins, J. R., Hirst, E., Kioussis, D., & Lovell Badge, R. (1996). Establishment and characterization of conditionally immortalized cells from the mouse urogenital ridge. Journal of Cell Science, 109 ( Pt 5), 899–909.
Capel, B., J. R. Hawkins, E. Hirst, D. Kioussis, and R. Lovell Badge. “Establishment and characterization of conditionally immortalized cells from the mouse urogenital ridge.Journal of Cell Science 109 ( Pt 5) (May 1996): 899–909.
Capel B, Hawkins JR, Hirst E, Kioussis D, Lovell Badge R. Establishment and characterization of conditionally immortalized cells from the mouse urogenital ridge. Journal of cell science. 1996 May;109 ( Pt 5):899–909.
Capel, B., et al. “Establishment and characterization of conditionally immortalized cells from the mouse urogenital ridge.Journal of Cell Science, vol. 109 ( Pt 5), May 1996, pp. 899–909.
Capel B, Hawkins JR, Hirst E, Kioussis D, Lovell Badge R. Establishment and characterization of conditionally immortalized cells from the mouse urogenital ridge. Journal of cell science. 1996 May;109 ( Pt 5):899–909.
Journal cover image

Published In

Journal of cell science

ISSN

0021-9533

Publication Date

May 1996

Volume

109 ( Pt 5)

Start / End Page

899 / 909

Location

england

Related Subject Headings

  • Urogenital System
  • Temperature
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Male
  • Germ Cells
  • Female
  • Extracellular Matrix
  • Developmental Biology