Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Sox8 is expressed at similar levels in gonads of both sexes during the sex determining period in turtles.

Publication ,  Journal Article
Takada, S; DiNapoli, L; Capel, B; Koopman, P
Published in: Dev Dyn
October 2004

A critical gene involved in mammalian sex determination and differentiation is the Sry-related gene Sox9. In reptiles, Sox9 resembles that of mammals in both structure and expression pattern in the developing gonad, but a causal role in male sex determination has not been established. A closely related gene, Sox8, is conserved in human, mouse, and trout and is expressed in developing testes and not developing ovaries in mouse. In this study, we tested the possibility of Sox8 being important for sex determination or sex differentiation in the red-eared slider turtle Trachemys scripta, in which sex is determined by egg incubation temperature between stages 15 and 20. We cloned partial turtle Sox8 and anti-Müllerian hormone (Amh) cDNAs, and analyzed the expression patterns of these genes in developing gonads by reverse transcriptase-polymerase chain reaction and whole-mount in situ hybridization. While Amh is expressed more strongly in males than in females at stage 17, Sox8 is expressed at similar levels in males and females throughout the sex-determining period. These observations suggest that differential transcription of Sox8 is not responsible for regulation of Amh, nor responsible for sex determination in turtle.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Dev Dyn

DOI

ISSN

1058-8388

Publication Date

October 2004

Volume

231

Issue

2

Start / End Page

387 / 395

Location

United States

Related Subject Headings

  • Turtles
  • Transcription Factors
  • Testicular Hormones
  • Sex Differentiation
  • Sex Determination Processes
  • Sequence Alignment
  • SOXE Transcription Factors
  • Molecular Sequence Data
  • Mice
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Takada, S., DiNapoli, L., Capel, B., & Koopman, P. (2004). Sox8 is expressed at similar levels in gonads of both sexes during the sex determining period in turtles. Dev Dyn, 231(2), 387–395. https://doi.org/10.1002/dvdy.20132
Takada, Shuji, Leo DiNapoli, Blanche Capel, and Peter Koopman. “Sox8 is expressed at similar levels in gonads of both sexes during the sex determining period in turtles.Dev Dyn 231, no. 2 (October 2004): 387–95. https://doi.org/10.1002/dvdy.20132.
Takada S, DiNapoli L, Capel B, Koopman P. Sox8 is expressed at similar levels in gonads of both sexes during the sex determining period in turtles. Dev Dyn. 2004 Oct;231(2):387–95.
Takada, Shuji, et al. “Sox8 is expressed at similar levels in gonads of both sexes during the sex determining period in turtles.Dev Dyn, vol. 231, no. 2, Oct. 2004, pp. 387–95. Pubmed, doi:10.1002/dvdy.20132.
Takada S, DiNapoli L, Capel B, Koopman P. Sox8 is expressed at similar levels in gonads of both sexes during the sex determining period in turtles. Dev Dyn. 2004 Oct;231(2):387–395.
Journal cover image

Published In

Dev Dyn

DOI

ISSN

1058-8388

Publication Date

October 2004

Volume

231

Issue

2

Start / End Page

387 / 395

Location

United States

Related Subject Headings

  • Turtles
  • Transcription Factors
  • Testicular Hormones
  • Sex Differentiation
  • Sex Determination Processes
  • Sequence Alignment
  • SOXE Transcription Factors
  • Molecular Sequence Data
  • Mice
  • Male