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Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue.

Publication ,  Journal Article
O'Brien, SN; Mantzke, KA; Kilgore, MW; Price, TM
Published in: Anal Quant Cytol Histol
April 1996
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OBJECTIVE: To determine the relative amounts of RNA and DNA from adipocytes and adipose stromal-vascular cells to better assess differential gene expression for estrogen receptor and cytochrome P450 aromatase in the two cell fractions of adipose tissue. STUDY DESIGN: Colorimetric and fluorometric assays were used to measure total RNA and DNA, and the relative cell numbers as well as the relative contribution of RNA from adipocytes and stromal-vascular cells were assessed. RESULTS: Adipocytes and stromal-vascular cells exist in a ratio of 2/1. RNA/DNA ratios are the same in the two cell fractions. CONCLUSION: In human subcutaneous adipose tissue, adipocytes are twice as numerous as stromal-vascular cells. The overall transcriptional activity of the cell types appears similar.

Duke Scholars

Thomas Michael Price
Author Thomas Michael Price Obstetrics and Gynecology, Reproductive Endocrinology & Infe ...

Published In

Anal Quant Cytol Histol

Publication Date

April 1996

Volume

18

Issue

2

Start / End Page

137 / 143

Location

United States

Related Subject Headings

  • Stromal Cells
  • Receptors, Estrogen
  • RNA
  • Pathology
  • Oncology & Carcinogenesis
  • Middle Aged
  • Humans
  • Gene Expression
  • Fluorometry
  • Female
 

Citation

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O’Brien, S. N., Mantzke, K. A., Kilgore, M. W., & Price, T. M. (1996). Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue. Anal Quant Cytol Histol, 18(2), 137–143.
O’Brien, S. N., K. A. Mantzke, M. W. Kilgore, and T. M. Price. “Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue.” Anal Quant Cytol Histol 18, no. 2 (April 1996): 137–43.
O’Brien SN, Mantzke KA, Kilgore MW, Price TM. Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue. Anal Quant Cytol Histol. 1996 Apr;18(2):137–43.
O’Brien, S. N., et al. “Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue.” Anal Quant Cytol Histol, vol. 18, no. 2, Apr. 1996, pp. 137–43.
O’Brien SN, Mantzke KA, Kilgore MW, Price TM. Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue. Anal Quant Cytol Histol. 1996 Apr;18(2):137–143.

Published In

Anal Quant Cytol Histol

Publication Date

April 1996

Volume

18

Issue

2

Start / End Page

137 / 143

Location

United States

Related Subject Headings

  • Stromal Cells
  • Receptors, Estrogen
  • RNA
  • Pathology
  • Oncology & Carcinogenesis
  • Middle Aged
  • Humans
  • Gene Expression
  • Fluorometry
  • Female