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Mitochondrial oxidant production by a pollutant dust and NO-mediated apoptosis in human alveolar macrophage.

Publication ,  Journal Article
Huang, Y-CT; Soukup, J; Harder, S; Becker, S
Published in: Am J Physiol Cell Physiol
January 2003

Residual oil fly ash (ROFA) is a pollutant dust that stimulates production of reactive oxygen species (ROS) from mitochondria and apoptosis in alveolar macrophages (AM), but the relationship between these two processes is unclear. In this study, human AM were incubated with ROFA or vanadyl sulfate (VOSO(4)), the major metal constituent in ROFA, with or without nitro-L-arginine methyl ester (L-NAME), diphenyleneiodonium (DPI), and mitochondrial electron transport inhibitors. Interactions among production of ROS, nitric oxide (NO), and apoptosis of AM were determined. ROFA-stimulated ROS production was attenuated by DPI, rotenone, antimycin, and NaN(3), but not by L-NAME, a pattern mimicked by VOSO(4). ROFA-induced apoptosis was inhibited by L-NAME and a caspase-3-like protease inhibitor, but not by mitochondrial inhibitors. ROFA enhanced NO-mediated increase in caspase-3-like activity. VOSO(4) had minor effects on apoptosis. Thus ROFA-stimulated production of ROS from mitochondria was independent of apoptosis of AM, which was mediated by activation of caspase-3-like proteases and NO. The pro-oxidant effect but not the proapoptotic effect of ROFA was mediated by vanadium.

Duke Scholars

Published In

Am J Physiol Cell Physiol

DOI

ISSN

0363-6143

Publication Date

January 2003

Volume

284

Issue

1

Start / End Page

C24 / C32

Location

United States

Related Subject Headings

  • Vanadium
  • Reactive Oxygen Species
  • Physiology
  • Particulate Matter
  • Oxidants
  • Nitric Oxide
  • NG-Nitroarginine Methyl Ester
  • Mitochondria
  • Macrophages, Alveolar
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
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Huang, Y.-C., Soukup, J., Harder, S., & Becker, S. (2003). Mitochondrial oxidant production by a pollutant dust and NO-mediated apoptosis in human alveolar macrophage. Am J Physiol Cell Physiol, 284(1), C24–C32. https://doi.org/10.1152/ajpcell.00139.2002
Huang, Yuh-Chin T., Joleen Soukup, Shirley Harder, and Susanne Becker. “Mitochondrial oxidant production by a pollutant dust and NO-mediated apoptosis in human alveolar macrophage.Am J Physiol Cell Physiol 284, no. 1 (January 2003): C24–32. https://doi.org/10.1152/ajpcell.00139.2002.
Huang Y-CT, Soukup J, Harder S, Becker S. Mitochondrial oxidant production by a pollutant dust and NO-mediated apoptosis in human alveolar macrophage. Am J Physiol Cell Physiol. 2003 Jan;284(1):C24–32.
Huang, Yuh-Chin T., et al. “Mitochondrial oxidant production by a pollutant dust and NO-mediated apoptosis in human alveolar macrophage.Am J Physiol Cell Physiol, vol. 284, no. 1, Jan. 2003, pp. C24–32. Pubmed, doi:10.1152/ajpcell.00139.2002.
Huang Y-CT, Soukup J, Harder S, Becker S. Mitochondrial oxidant production by a pollutant dust and NO-mediated apoptosis in human alveolar macrophage. Am J Physiol Cell Physiol. 2003 Jan;284(1):C24–C32.

Published In

Am J Physiol Cell Physiol

DOI

ISSN

0363-6143

Publication Date

January 2003

Volume

284

Issue

1

Start / End Page

C24 / C32

Location

United States

Related Subject Headings

  • Vanadium
  • Reactive Oxygen Species
  • Physiology
  • Particulate Matter
  • Oxidants
  • Nitric Oxide
  • NG-Nitroarginine Methyl Ester
  • Mitochondria
  • Macrophages, Alveolar
  • Humans