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Expression of a constitutive form of calcium/calmodulin dependent protein kinase II leads to arrest of the cell cycle in G2.

Publication ,  Journal Article
Planas-Silva, MD; Means, AR
Published in: EMBO J
February 1992

Calcium/calmodulin dependent protein kinase II (CaMKII) is a multifunctional serine/threonine protein kinase. We have created a calcium/calmodulin independent form of this enzyme by truncation. Expression of this enzyme fragment in a rabbit reticulocyte lysate yields a constitutive enzyme with specific activity similar to the activated native enzyme. We have established mammalian cell lines that transiently express this constitutive enzyme using the glucocorticoid-inducible mouse mammary tumor virus long terminal repeat. The transient increase in kinase activity results in a complete cessation of cell cycle progression. This block develops as a consequence of a specific arrest of the cell cycle in G2. During the block, increases in histone H1 kinase activity present in p13 beads or anti-cdc2 immunoprecipitates are seen in parallel with the accumulation of cells at G2, arguing that the arrest is not due to a failure to activate cdc2 as a histone H1 kinase. These results suggest that other changes in serine/threonine protein phosphorylation besides those involved in activation of cdc2 as a histone H1 kinase may be necessary for proper G2-M transition.

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Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

February 1992

Volume

11

Issue

2

Start / End Page

507 / 517

Location

England

Related Subject Headings

  • Transfection
  • Transcription, Genetic
  • Repetitive Sequences, Nucleic Acid
  • RNA
  • Protein Kinases
  • Protein Biosynthesis
  • Protamine Kinase
  • Plasmids
  • Molecular Sequence Data
  • Mice
 

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Planas-Silva, M. D., & Means, A. R. (1992). Expression of a constitutive form of calcium/calmodulin dependent protein kinase II leads to arrest of the cell cycle in G2. EMBO J, 11(2), 507–517. https://doi.org/10.1002/j.1460-2075.1992.tb05081.x
Planas-Silva, M. D., and A. R. Means. “Expression of a constitutive form of calcium/calmodulin dependent protein kinase II leads to arrest of the cell cycle in G2.EMBO J 11, no. 2 (February 1992): 507–17. https://doi.org/10.1002/j.1460-2075.1992.tb05081.x.
Planas-Silva, M. D., and A. R. Means. “Expression of a constitutive form of calcium/calmodulin dependent protein kinase II leads to arrest of the cell cycle in G2.EMBO J, vol. 11, no. 2, Feb. 1992, pp. 507–17. Pubmed, doi:10.1002/j.1460-2075.1992.tb05081.x.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

February 1992

Volume

11

Issue

2

Start / End Page

507 / 517

Location

England

Related Subject Headings

  • Transfection
  • Transcription, Genetic
  • Repetitive Sequences, Nucleic Acid
  • RNA
  • Protein Kinases
  • Protein Biosynthesis
  • Protamine Kinase
  • Plasmids
  • Molecular Sequence Data
  • Mice