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Calcium-dependent regulator protein: localization in mitotic apparatus of eukaryotic cells.

Publication ,  Journal Article
Welsh, MJ; Dedman, JR; Brinkley, BR; Means, AR
Published in: Proc Natl Acad Sci U S A
April 1978

Calcium-dependent regulator protein is a low molecular weight (17,000), thermostable, calcium binding protein which is structurally homologous to skeletal muscle troponin C. This protein is present in all nonmuscle cells and has been shown to decorate stress fibers in interphase cells by indirect immunofluorescence. Using this procedure we have investigated the distribution of the protein during mitosis of eukaryotic cells. As the cells enter prophase, the distinct cytoplasmic localization disappears commensurate with the dissolution of the cytoskeleton. The regulator protein seems to be randomly distributed throughout the prophase cell, including the region around the condensed chromosomes. However, at prometaphase, it is localized in association with the half-spindles of the mitotic apparatus. Through metaphase and most of anaphase, the protein remains localized between the chromosomes and the poles of the spindle. During late anaphase the protein is also found in the interzone region but rapidly condenses into two small regions, one on each side of the midbody that separates the daughter cells. The regulator protein is not localized in the cleavage furrow during telophase, whereas actin is demonstrable in this region. Indeed, placement of the protein during mitosis is distinct from both that of actin and that of tubulin. The localization of calcium-dependent regulator protein during mitosis suggests that it may mediate the calcium effects on the mitotic apparatus and thus play a role in chromosome movement.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 1978

Volume

75

Issue

4

Start / End Page

1867 / 1871

Location

United States

Related Subject Headings

  • Telophase
  • Prophase
  • Organoids
  • Mitosis
  • Microtubules
  • Metaphase
  • Interphase
  • Cell Line
  • Carrier Proteins
  • Calcium
 

Citation

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Welsh, M. J., Dedman, J. R., Brinkley, B. R., & Means, A. R. (1978). Calcium-dependent regulator protein: localization in mitotic apparatus of eukaryotic cells. Proc Natl Acad Sci U S A, 75(4), 1867–1871. https://doi.org/10.1073/pnas.75.4.1867
Welsh, M. J., J. R. Dedman, B. R. Brinkley, and A. R. Means. “Calcium-dependent regulator protein: localization in mitotic apparatus of eukaryotic cells.Proc Natl Acad Sci U S A 75, no. 4 (April 1978): 1867–71. https://doi.org/10.1073/pnas.75.4.1867.
Welsh MJ, Dedman JR, Brinkley BR, Means AR. Calcium-dependent regulator protein: localization in mitotic apparatus of eukaryotic cells. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1867–71.
Welsh, M. J., et al. “Calcium-dependent regulator protein: localization in mitotic apparatus of eukaryotic cells.Proc Natl Acad Sci U S A, vol. 75, no. 4, Apr. 1978, pp. 1867–71. Pubmed, doi:10.1073/pnas.75.4.1867.
Welsh MJ, Dedman JR, Brinkley BR, Means AR. Calcium-dependent regulator protein: localization in mitotic apparatus of eukaryotic cells. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1867–1871.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 1978

Volume

75

Issue

4

Start / End Page

1867 / 1871

Location

United States

Related Subject Headings

  • Telophase
  • Prophase
  • Organoids
  • Mitosis
  • Microtubules
  • Metaphase
  • Interphase
  • Cell Line
  • Carrier Proteins
  • Calcium