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Molecular cloning sequence and distribution of rat calspermin, a high affinity calmodulin-binding protein.

Publication ,  Journal Article
Ono, T; Slaughter, GR; Cook, RG; Means, AR
Published in: J Biol Chem
February 5, 1989

Calspermin is a heat-stable, acidic calmodulin-binding protein predominantly found in mammalian testis. The cDNA representing the rat form of this protein has been cloned from a rat testis lambda gt11 library. Sequence analysis of two overlapping clones revealed a 232-nucleotide 5'-nontranslated region, 510 nucleotides of open reading frame, a 148-nucleotide 3'-untranslated region, and a poly(A) tail. Authenticity of the clones was confirmed by comparison of a portion of the deduced amino acid sequence with the sequence of a tryptic peptide obtained from the rat testis protein. The lambda gt11 fusion protein was recognized by affinity purified antibodies to pig testis calspermin and bound 125I-calmodulin in a Ca2+-dependent manner. Calspermin cDNA encodes a 169-residue protein with a calculated Mr of 18,735. The putative calmodulin-binding domain is very close to the amino terminus of the protein. This region shows 46% identity with the calmodulin-binding region of rat brain Ca2+/calmodulin-dependent protein kinase II and 32% identity with the equivalent region of chicken smooth muscle myosin light chain kinase. The 5'-nontranslated region reveals significant homology with a portion of the catalytic region of the calmodulin-dependent protein kinase family. Calspermin contains a stretch of 17 contiguous glutamic acid residues in the central region of the molecule. Computer analysis predicts calspermin to be 81% alpha-helix and 14% random coil. Analysis of genomic DNA indicates calspermin to be the product of a unique gene. Northern blot analysis of rat testis RNA reveals a 1.1-kilobase mRNA. This RNA is restricted to testis among several rat tissues examined and could not be identified in total RNA isolated from testes of other mammals. Analysis of cells isolated from rat testis reveals calspermin mRNA to be predominantly expressed in postmeiotic cells indicating that it may be specific to haploid cells.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

February 5, 1989

Volume

264

Issue

4

Start / End Page

2081 / 2087

Location

United States

Related Subject Headings

  • Testis
  • Species Specificity
  • Sequence Homology, Nucleic Acid
  • Rats
  • Organ Specificity
  • Molecular Sequence Data
  • Male
  • Genes
  • Cloning, Molecular
  • Calmodulin-Binding Proteins
 

Citation

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Ono, T., Slaughter, G. R., Cook, R. G., & Means, A. R. (1989). Molecular cloning sequence and distribution of rat calspermin, a high affinity calmodulin-binding protein. J Biol Chem, 264(4), 2081–2087.
Ono, T., G. R. Slaughter, R. G. Cook, and A. R. Means. “Molecular cloning sequence and distribution of rat calspermin, a high affinity calmodulin-binding protein.J Biol Chem 264, no. 4 (February 5, 1989): 2081–87.
Ono T, Slaughter GR, Cook RG, Means AR. Molecular cloning sequence and distribution of rat calspermin, a high affinity calmodulin-binding protein. J Biol Chem. 1989 Feb 5;264(4):2081–7.
Ono, T., et al. “Molecular cloning sequence and distribution of rat calspermin, a high affinity calmodulin-binding protein.J Biol Chem, vol. 264, no. 4, Feb. 1989, pp. 2081–87.
Ono T, Slaughter GR, Cook RG, Means AR. Molecular cloning sequence and distribution of rat calspermin, a high affinity calmodulin-binding protein. J Biol Chem. 1989 Feb 5;264(4):2081–2087.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

February 5, 1989

Volume

264

Issue

4

Start / End Page

2081 / 2087

Location

United States

Related Subject Headings

  • Testis
  • Species Specificity
  • Sequence Homology, Nucleic Acid
  • Rats
  • Organ Specificity
  • Molecular Sequence Data
  • Male
  • Genes
  • Cloning, Molecular
  • Calmodulin-Binding Proteins