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cAMP-response element modulator tau is a positive regulator of testis angiotensin converting enzyme transcription.

Publication ,  Journal Article
Zhou, Y; Sun, Z; Means, AR; Sassone-Corsi, P; Bernstein, KE
Published in: Proc Natl Acad Sci U S A
October 29, 1996

Testis angiotensin-converting enzyme (ACE) is a unique form of ACE, only produced by male germ cells, and results from a testis-specific promoter found within the ACE gene. We have investigated the role of cAMP-response element modulator (CREM)tau in testis ACE transcription. In gel shift experiments, testes nuclear proteins retard an oligonucleotide containing the cAMP-response element (CRE) found at position -55 in the testis ACE promoter. Anti-CREM antibody supershifts this complex. Competitive gel shift shows that recombinant CREM tau protein and testis nuclear proteins have a similar specificity of binding to the tests ACE CRE. Functional analysis using in vitro transcription and transfection studies also demonstrate that CREM tau protein is a transcriptional activator of the testis ACE promoter. Western blot analysis identifies CREM tau protein in the protein-DNA complex formed between nuclear proteins and the testis ACE CRE motif. This analysis also identified other CREM isoforms in the gel-shifted complex, which are thought to be CREM tau 1/2, CREM alpha/beta, and S-CREM. These data indicate that CREM tau isoforms play an important role as a positive regulator in the tissue-specific expression of testis ACE.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 29, 1996

Volume

93

Issue

22

Start / End Page

12262 / 12266

Location

United States

Related Subject Headings

  • Transfection
  • Testis
  • Spermatogenesis
  • Repressor Proteins
  • Rats, Sprague-Dawley
  • Rats
  • Peptidyl-Dipeptidase A
  • Male
  • Humans
  • Gene Expression Regulation, Enzymologic
 

Citation

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Zhou, Y., Sun, Z., Means, A. R., Sassone-Corsi, P., & Bernstein, K. E. (1996). cAMP-response element modulator tau is a positive regulator of testis angiotensin converting enzyme transcription. Proc Natl Acad Sci U S A, 93(22), 12262–12266. https://doi.org/10.1073/pnas.93.22.12262
Zhou, Y., Z. Sun, A. R. Means, P. Sassone-Corsi, and K. E. Bernstein. “cAMP-response element modulator tau is a positive regulator of testis angiotensin converting enzyme transcription.Proc Natl Acad Sci U S A 93, no. 22 (October 29, 1996): 12262–66. https://doi.org/10.1073/pnas.93.22.12262.
Zhou Y, Sun Z, Means AR, Sassone-Corsi P, Bernstein KE. cAMP-response element modulator tau is a positive regulator of testis angiotensin converting enzyme transcription. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12262–6.
Zhou, Y., et al. “cAMP-response element modulator tau is a positive regulator of testis angiotensin converting enzyme transcription.Proc Natl Acad Sci U S A, vol. 93, no. 22, Oct. 1996, pp. 12262–66. Pubmed, doi:10.1073/pnas.93.22.12262.
Zhou Y, Sun Z, Means AR, Sassone-Corsi P, Bernstein KE. cAMP-response element modulator tau is a positive regulator of testis angiotensin converting enzyme transcription. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12262–12266.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 29, 1996

Volume

93

Issue

22

Start / End Page

12262 / 12266

Location

United States

Related Subject Headings

  • Transfection
  • Testis
  • Spermatogenesis
  • Repressor Proteins
  • Rats, Sprague-Dawley
  • Rats
  • Peptidyl-Dipeptidase A
  • Male
  • Humans
  • Gene Expression Regulation, Enzymologic