Domain II of calmodulin is involved in activation of calcineurin.
Publication
, Journal Article
Hurwitz, MY; Putkey, JA; Klee, CB; Means, AR
Published in: FEBS Lett
September 26, 1988
A family of mutant proteins related to calmodulin (CaM) has been produced using cDNA constructs in bacterial expression vectors. The new proteins contain amino acid substitutions in Ca2+-binding domains I, II, both I and II, or both II and IV. The calmodulin-like proteins have been characterized with respect to mobility on SDS-polyacrylamide gels, Ca2+-dependent enhancement of tyrosine fluorescence, and abilities to activate the CaM-dependent phosphatase calcineurin. These studies suggest that an intact Ca2+-binding domain II is minimally required for full activation of calcineurin.
Duke Scholars
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
September 26, 1988
Volume
238
Issue
1
Start / End Page
82 / 86
Location
England
Related Subject Headings
- Pseudogenes
- Phosphoprotein Phosphatases
- Genetic Vectors
- Genes
- Enzyme Activation
- Chickens
- Calmodulin-Binding Proteins
- Calmodulin
- Calcineurin
- Biochemistry & Molecular Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Hurwitz, M. Y., Putkey, J. A., Klee, C. B., & Means, A. R. (1988). Domain II of calmodulin is involved in activation of calcineurin. FEBS Lett, 238(1), 82–86. https://doi.org/10.1016/0014-5793(88)80230-8
Hurwitz, M. Y., J. A. Putkey, C. B. Klee, and A. R. Means. “Domain II of calmodulin is involved in activation of calcineurin.” FEBS Lett 238, no. 1 (September 26, 1988): 82–86. https://doi.org/10.1016/0014-5793(88)80230-8.
Hurwitz MY, Putkey JA, Klee CB, Means AR. Domain II of calmodulin is involved in activation of calcineurin. FEBS Lett. 1988 Sep 26;238(1):82–6.
Hurwitz, M. Y., et al. “Domain II of calmodulin is involved in activation of calcineurin.” FEBS Lett, vol. 238, no. 1, Sept. 1988, pp. 82–86. Pubmed, doi:10.1016/0014-5793(88)80230-8.
Hurwitz MY, Putkey JA, Klee CB, Means AR. Domain II of calmodulin is involved in activation of calcineurin. FEBS Lett. 1988 Sep 26;238(1):82–86.
Published In
FEBS Lett
DOI
ISSN
0014-5793
Publication Date
September 26, 1988
Volume
238
Issue
1
Start / End Page
82 / 86
Location
England
Related Subject Headings
- Pseudogenes
- Phosphoprotein Phosphatases
- Genetic Vectors
- Genes
- Enzyme Activation
- Chickens
- Calmodulin-Binding Proteins
- Calmodulin
- Calcineurin
- Biochemistry & Molecular Biology