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The effect of antibodies and immunotoxins reactive with HER-2/neu on growth of ovarian and breast cancer cell lines.

Publication ,  Journal Article
Rodríguez, GC; Boente, MP; Berchuck, A; Whitaker, RS; O'Briant, KC; Xu, F; Bast, RC
Published in: Am J Obstet Gynecol
January 1993

OBJECTIVE: Because HER-2/neu is overexpressed in one third of breast and ovarian cancers, we examined the effect of unconjugated monoclonal antibodies (ID-5, PB-3, TA-1) and an immunotoxin (TA-1-ricin) reactive with this protooncogene on the growth of breast and ovarian cancer cell lines. STUDY DESIGN: The tritiated thymidine incorporation assay was used to examine the effect of unconjugated antibodies on proliferation. A limiting dilution clonogenic assay was used to assess the effect of immunotoxin on cellular cytotoxicity. RESULTS: Scatchard analysis revealed that OVCA 420, OVCA 429, OVCA 432, and OVCA 433 cells had approximately 10(4) HER-2/neu receptors per cell, whereas the SKOv3 and SKBr3 cell lines expressed 10(5) and 10(6) receptors per cell, respectively. Monoclonal antibody ID-5 caused significant inhibition of tritiated thymidine incorporation in SKBr3, SKOv3, and OVCA 420 cells (p < 0.002). The TA-1-rich immunotoxin significantly inhibited the clonogenic growth of only SKBr3 and SKOv3 cells. CONCLUSION: HER-2/neu may be a useful target for immunotherapy with unconjugated antibodies and immunotoxins in ovarian and breast cancers that overexpress this protooncogene.

Duke Scholars

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

January 1993

Volume

168

Issue

1 Pt 1

Start / End Page

228 / 232

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Ricin
  • Proto-Oncogenes
  • Proto-Oncogene Proteins
  • Ovarian Neoplasms
  • Obstetrics & Reproductive Medicine
  • Immunotherapy
  • Humans
  • Gene Expression
  • Female
 

Citation

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Chicago
ICMJE
MLA
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Rodríguez, G. C., Boente, M. P., Berchuck, A., Whitaker, R. S., O’Briant, K. C., Xu, F., & Bast, R. C. (1993). The effect of antibodies and immunotoxins reactive with HER-2/neu on growth of ovarian and breast cancer cell lines. Am J Obstet Gynecol, 168(1 Pt 1), 228–232. https://doi.org/10.1016/s0002-9378(12)90918-7
Rodríguez, G. C., M. P. Boente, A. Berchuck, R. S. Whitaker, K. C. O’Briant, F. Xu, and R. C. Bast. “The effect of antibodies and immunotoxins reactive with HER-2/neu on growth of ovarian and breast cancer cell lines.Am J Obstet Gynecol 168, no. 1 Pt 1 (January 1993): 228–32. https://doi.org/10.1016/s0002-9378(12)90918-7.
Rodríguez GC, Boente MP, Berchuck A, Whitaker RS, O’Briant KC, Xu F, et al. The effect of antibodies and immunotoxins reactive with HER-2/neu on growth of ovarian and breast cancer cell lines. Am J Obstet Gynecol. 1993 Jan;168(1 Pt 1):228–32.
Rodríguez, G. C., et al. “The effect of antibodies and immunotoxins reactive with HER-2/neu on growth of ovarian and breast cancer cell lines.Am J Obstet Gynecol, vol. 168, no. 1 Pt 1, Jan. 1993, pp. 228–32. Pubmed, doi:10.1016/s0002-9378(12)90918-7.
Rodríguez GC, Boente MP, Berchuck A, Whitaker RS, O’Briant KC, Xu F, Bast RC. The effect of antibodies and immunotoxins reactive with HER-2/neu on growth of ovarian and breast cancer cell lines. Am J Obstet Gynecol. 1993 Jan;168(1 Pt 1):228–232.
Journal cover image

Published In

Am J Obstet Gynecol

DOI

ISSN

0002-9378

Publication Date

January 1993

Volume

168

Issue

1 Pt 1

Start / End Page

228 / 232

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Ricin
  • Proto-Oncogenes
  • Proto-Oncogene Proteins
  • Ovarian Neoplasms
  • Obstetrics & Reproductive Medicine
  • Immunotherapy
  • Humans
  • Gene Expression
  • Female