Class I restricted CTL recognition of a soluble protein delivered by liposomes containing lipophilic polylysines.
CD8+ cytotoxic lymphocytes recognize peptides derived from endogenous antigens complexed with class I major histocompatibility complex while CD4+ helper cells recognize peptides from exogenous antigens bound to class II MHC molecules. A soluble protein can be introduced into the class I pathway of antigen processing and presentation using an appropriate vehicle to deliver the antigen into the cytosol. Cationic liposomes containing lipophilic polylysine readily form complexes with an anionic, soluble protein ovalbumin. Mouse thymoma EL4 cells incubated with such complexes can be sensitized for killing by OVA-specific CTL effector cells. This method of target sensitization by a soluble antigen is more sensitive than the osmotic loading method previously reported.
Duke Scholars
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Related Subject Headings
- Tumor Cells, Cultured
- Tetradecanoylphorbol Acetate
- T-Lymphocytes, Regulatory
- T-Lymphocytes, Cytotoxic
- Spleen
- Polylysine
- Ovalbumin
- Mice, Inbred C57BL
- Mice
- Liposomes
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tumor Cells, Cultured
- Tetradecanoylphorbol Acetate
- T-Lymphocytes, Regulatory
- T-Lymphocytes, Cytotoxic
- Spleen
- Polylysine
- Ovalbumin
- Mice, Inbred C57BL
- Mice
- Liposomes