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Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo.

Publication ,  Journal Article
Boczkowski, D; Nair, SK; Snyder, D; Gilboa, E
Published in: J Exp Med
August 1, 1996

Immunization with defined tumor antigens is currently limited to a small number of cancers where candidates for tumor rejection antigens have been identified. In this study we investigated whether pulsing dendritic cells (DC) with tumor-derived RNA is an effective way to induce CTL and tumor immunity. DC pulsed with in vitro synthesized chicken ovalbumin (OVA) RNA were more effective than OVA peptide-pulsed DC in stimulating primary, OVA-specific CTL responses in vitro. DC pulsed with unfractionated RNA (total or polyA+) from OVA-expressing tumor cells were as effective as DC pulsed with OVA peptide at stimulating CTL responses. Induction of OVA-specific CTL was abrogated when polyA+ RNA from OVA-expressing cells was treated with an OVA-specific antisense oligodeoxynucleotide and RNase H, showing that sensitization of DC was indeed mediated by OVA RNA. Mice vaccinated with DC pulsed with RNA from OVA-expressing tumor cells were protected against a challenge with OVA-expressing tumor cells. In the poorly immunogenic, highly metastatic, B16/F10.9 tumor model a dramatic reduction in lung metastases was observed in mice vaccinated with DC pulsed with tumor-derived RNA (total or polyA+, but not polyA- RNA). The finding that RNA transcribed in vitro from cDNA cloned in a bacterial plasmid was highly effective in sensitizing DC shows that amplification of the antigenic content from a small number of tumor cells is feasible, thus expanding the potential use of RNA-pulsed DC-based vaccines for patients bearing very small, possibly microscopic, tumors.

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Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

August 1, 1996

Volume

184

Issue

2

Start / End Page

465 / 472

Location

United States

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • RNA, Messenger
  • RNA
  • Ovalbumin
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • Immunity, Cellular
  • Dendritic Cells
 

Citation

APA
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ICMJE
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Boczkowski, D., Nair, S. K., Snyder, D., & Gilboa, E. (1996). Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo. J Exp Med, 184(2), 465–472. https://doi.org/10.1084/jem.184.2.465
Boczkowski, D., S. K. Nair, D. Snyder, and E. Gilboa. “Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo.J Exp Med 184, no. 2 (August 1, 1996): 465–72. https://doi.org/10.1084/jem.184.2.465.
Boczkowski D, Nair SK, Snyder D, Gilboa E. Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo. J Exp Med. 1996 Aug 1;184(2):465–72.
Boczkowski, D., et al. “Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo.J Exp Med, vol. 184, no. 2, Aug. 1996, pp. 465–72. Pubmed, doi:10.1084/jem.184.2.465.
Boczkowski D, Nair SK, Snyder D, Gilboa E. Dendritic cells pulsed with RNA are potent antigen-presenting cells in vitro and in vivo. J Exp Med. 1996 Aug 1;184(2):465–472.

Published In

J Exp Med

DOI

ISSN

0022-1007

Publication Date

August 1, 1996

Volume

184

Issue

2

Start / End Page

465 / 472

Location

United States

Related Subject Headings

  • T-Lymphocytes, Cytotoxic
  • RNA, Messenger
  • RNA
  • Ovalbumin
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • Immunity, Cellular
  • Dendritic Cells