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The Mycobacterium tuberculosis 71-kDa heat-shock protein induces proliferation and cytokine secretion by murine gut intraepithelial lymphocytes.

Publication ,  Journal Article
Beagley, KW; Fujihashi, K; Black, CA; Lagoo, AS; Yamamoto, M; McGhee, JR; Kiyono, H
Published in: Eur J Immunol
August 1993

Murine intraepithelial lymphocytes (IEL) respond poorly to T cell mitogens and to monoclonal antibody stimulation of T cell receptor (TCR)- and CD3- associated molecules. In contrast, we found that a soluble extract of Mycobacterium tuberculosis (Mtb), but not purified protein derivative of tuberculin, induced significant proliferative responses in IEL cultures. The active component was apparently a heat shock protein (HSP), since recombinant 71-kDa HSP from Mtb induced IEL to proliferate, while 65-kDa HSP from M. bovis and M. leprae did not. Both alpha/beta and gamma/delta TCR-enriched IEL gave proliferative responses to 71-kDa HSP. Further, culture supernatants from IEL stimulated with 71-kDa HSP contained elevated levels of interleukin-(IL)-3/granulocyte-macrophage colony-stimulating factor, interferon-gamma and IL-6, but not IL-2, IL-4, IL-5 or transforming growth factor-beta. Finally, several IEL T cell clones have been maintained for up to 6 weeks, when stimulated with 71-kDa HSP, IL-2 and feeder cells. Our results show that the 71-kDa HSP of Mtb induces IEL T cells to divide and to secrete cytokines and this may offer a model for cloning and study of IEL T cells in vitro.

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Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

August 1993

Volume

23

Issue

8

Start / End Page

2049 / 2052

Location

Germany

Related Subject Headings

  • Mycobacterium tuberculosis
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice, Inbred BALB C
  • Mice
  • Lymphocyte Activation
  • Intestines
  • Interleukin-2
  • Immunology
  • Heat-Shock Proteins
 

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Beagley, K. W., Fujihashi, K., Black, C. A., Lagoo, A. S., Yamamoto, M., McGhee, J. R., & Kiyono, H. (1993). The Mycobacterium tuberculosis 71-kDa heat-shock protein induces proliferation and cytokine secretion by murine gut intraepithelial lymphocytes. Eur J Immunol, 23(8), 2049–2052. https://doi.org/10.1002/eji.1830230852
Beagley, K. W., K. Fujihashi, C. A. Black, A. S. Lagoo, M. Yamamoto, J. R. McGhee, and H. Kiyono. “The Mycobacterium tuberculosis 71-kDa heat-shock protein induces proliferation and cytokine secretion by murine gut intraepithelial lymphocytes.Eur J Immunol 23, no. 8 (August 1993): 2049–52. https://doi.org/10.1002/eji.1830230852.
Beagley KW, Fujihashi K, Black CA, Lagoo AS, Yamamoto M, McGhee JR, et al. The Mycobacterium tuberculosis 71-kDa heat-shock protein induces proliferation and cytokine secretion by murine gut intraepithelial lymphocytes. Eur J Immunol. 1993 Aug;23(8):2049–52.
Beagley, K. W., et al. “The Mycobacterium tuberculosis 71-kDa heat-shock protein induces proliferation and cytokine secretion by murine gut intraepithelial lymphocytes.Eur J Immunol, vol. 23, no. 8, Aug. 1993, pp. 2049–52. Pubmed, doi:10.1002/eji.1830230852.
Beagley KW, Fujihashi K, Black CA, Lagoo AS, Yamamoto M, McGhee JR, Kiyono H. The Mycobacterium tuberculosis 71-kDa heat-shock protein induces proliferation and cytokine secretion by murine gut intraepithelial lymphocytes. Eur J Immunol. 1993 Aug;23(8):2049–2052.

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

August 1993

Volume

23

Issue

8

Start / End Page

2049 / 2052

Location

Germany

Related Subject Headings

  • Mycobacterium tuberculosis
  • Mice, Inbred C57BL
  • Mice, Inbred C3H
  • Mice, Inbred BALB C
  • Mice
  • Lymphocyte Activation
  • Intestines
  • Interleukin-2
  • Immunology
  • Heat-Shock Proteins