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Effect of contact time and force on monocyte adhesion to vascular endothelium.

Publication ,  Journal Article
Rinker, KD; Prabhakar, V; Truskey, GA
Published in: Biophysical journal
April 2001

In this study we examined whether monocytic cell attachment to vascular endothelium was affected by elevating shear stress at a constant shear rate. Contact time, which is inversely related to the shear rate, was fixed and viscosity elevated with dextran to increase the shear stress (and hence the net force on the cell) independently of shear rate. At a fixed contact time, tethering frequencies increased, rolling velocities decreased, and median arrest durations increased with increasing shear stress. Rolling and short arrests (< 0.2 s) were well fit by a single exponential consistent with adhesion via the formation of a single additional bond. The cell dissociation constant, k(off), increased when the shear stress was elevated at constant shear rate. Firmly adherent cells arresting for at least 0.2 s were well fit by a stochastic model involving dissociation from multiple bonds. Therefore, at a fixed contact time and increasing shear stress, bonds formed more frequently for rolling cells resulting in more short arrests, and more bonds formed for firmly arresting cells resulting in longer arrest durations. Possible mechanisms for this increased adhesion include greater monocyte deformation and/or more frequent penetration of microvilli through steric and charge barriers.

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Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

April 2001

Volume

80

Issue

4

Start / End Page

1722 / 1732

Related Subject Headings

  • Viscosity
  • Umbilical Veins
  • Time Factors
  • Stress, Mechanical
  • Monocytes
  • Models, Theoretical
  • Kinetics
  • Humans
  • Flow Cytometry
  • Endothelium, Vascular
 

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Rinker, K. D., Prabhakar, V., & Truskey, G. A. (2001). Effect of contact time and force on monocyte adhesion to vascular endothelium. Biophysical Journal, 80(4), 1722–1732. https://doi.org/10.1016/s0006-3495(01)76143-7
Rinker, K. D., V. Prabhakar, and G. A. Truskey. “Effect of contact time and force on monocyte adhesion to vascular endothelium.Biophysical Journal 80, no. 4 (April 2001): 1722–32. https://doi.org/10.1016/s0006-3495(01)76143-7.
Rinker KD, Prabhakar V, Truskey GA. Effect of contact time and force on monocyte adhesion to vascular endothelium. Biophysical journal. 2001 Apr;80(4):1722–32.
Rinker, K. D., et al. “Effect of contact time and force on monocyte adhesion to vascular endothelium.Biophysical Journal, vol. 80, no. 4, Apr. 2001, pp. 1722–32. Epmc, doi:10.1016/s0006-3495(01)76143-7.
Rinker KD, Prabhakar V, Truskey GA. Effect of contact time and force on monocyte adhesion to vascular endothelium. Biophysical journal. 2001 Apr;80(4):1722–1732.
Journal cover image

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

April 2001

Volume

80

Issue

4

Start / End Page

1722 / 1732

Related Subject Headings

  • Viscosity
  • Umbilical Veins
  • Time Factors
  • Stress, Mechanical
  • Monocytes
  • Models, Theoretical
  • Kinetics
  • Humans
  • Flow Cytometry
  • Endothelium, Vascular