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Adhesion and function of human endothelial cells co-cultured on smooth muscle cells.

Publication ,  Journal Article
Wallace, CS; Champion, JC; Truskey, GA
Published in: Annals of biomedical engineering
March 2007

To evaluate interactions between human endothelial cells (ECs) and smooth muscle cells (SMCs) for the development of tissue-engineered vessels, we examined the adhesion and key cell properties of human ECs grown on quiescent human aortic SMCs. ECs attached to SMCs spread more slowly than ECs attached to fibronectin surfaces, and ECs aligned along the direction of the SMCs. ECs attached firmly and less than 5% of the cells were removed by shear stresses as high as 300 dyn cm(-2). Unlike porcine SMCs and co-cultures, human SMCs or co-cultures do not contract under flow, and the human ECs and SMCs in co-culture align toward the direction of flow. A confluent endothelium could be maintained in co-culture for over 30 days, and some of the ECs reoriented perpendicular to the SMCs after 9 days in static culture. Surface tissue factor levels in ECs and SMCs were less in co-culture than in monoculture. Co-culture induced an increase in calponin expression in SMCs. These findings show that human co-cultures can be maintained for long culture periods, where the endothelium remains confluent and responds to long-term exposure to flow, and EC-SMC interactions lead to an increase in SMC differentiation and an EC surface that is less thrombotic.

Duke Scholars

Published In

Annals of biomedical engineering

DOI

EISSN

1573-9686

ISSN

0090-6964

Publication Date

March 2007

Volume

35

Issue

3

Start / End Page

375 / 386

Related Subject Headings

  • Swine
  • Myocytes, Smooth Muscle
  • Humans
  • Endothelial Cells
  • Coculture Techniques
  • Cell Adhesion
  • Biomedical Engineering
  • Animals
  • 4003 Biomedical engineering
  • 11 Medical and Health Sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Wallace, C. S., Champion, J. C., & Truskey, G. A. (2007). Adhesion and function of human endothelial cells co-cultured on smooth muscle cells. Annals of Biomedical Engineering, 35(3), 375–386. https://doi.org/10.1007/s10439-006-9230-5
Wallace, Charles Stevenson, John C. Champion, and George A. Truskey. “Adhesion and function of human endothelial cells co-cultured on smooth muscle cells.Annals of Biomedical Engineering 35, no. 3 (March 2007): 375–86. https://doi.org/10.1007/s10439-006-9230-5.
Wallace CS, Champion JC, Truskey GA. Adhesion and function of human endothelial cells co-cultured on smooth muscle cells. Annals of biomedical engineering. 2007 Mar;35(3):375–86.
Wallace, Charles Stevenson, et al. “Adhesion and function of human endothelial cells co-cultured on smooth muscle cells.Annals of Biomedical Engineering, vol. 35, no. 3, Mar. 2007, pp. 375–86. Epmc, doi:10.1007/s10439-006-9230-5.
Wallace CS, Champion JC, Truskey GA. Adhesion and function of human endothelial cells co-cultured on smooth muscle cells. Annals of biomedical engineering. 2007 Mar;35(3):375–386.
Journal cover image

Published In

Annals of biomedical engineering

DOI

EISSN

1573-9686

ISSN

0090-6964

Publication Date

March 2007

Volume

35

Issue

3

Start / End Page

375 / 386

Related Subject Headings

  • Swine
  • Myocytes, Smooth Muscle
  • Humans
  • Endothelial Cells
  • Coculture Techniques
  • Cell Adhesion
  • Biomedical Engineering
  • Animals
  • 4003 Biomedical engineering
  • 11 Medical and Health Sciences