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Role of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow

Publication ,  Journal Article
Iuliano, DJ; Proulx, TL; Saavedra, SS; Truskey, GA
Published in: American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
December 1, 1991

Forces exerted on adherent calls by fluid shear stresses can cause cell detachment. In this study, we examined the effect of cell spreading, actin fiber density, and the conformation of adsorbed adhesion proteins in promoting cell adhesion after a brief exposure to shear stresses between 5-80 dyne/cm2 in a parallel plate flow channel. Cell spreading led to increased adhesion. Cell spreading and adhesion were greater when the surface contained preadsorbed fibronectin. Spreading was accompanied by formation of cytoskeleton which strengthened the adhesive contact between the cell and surface. Similar numbers of cells were detached from hydrophobic and hydrophilic glass containing preadsorbed in even though the cell binding domain was in different conformations.

Duke Scholars

Published In

American Society of Mechanical Engineers, Bioengineering Division (Publication) BED

Publication Date

December 1, 1991

Volume

21

Start / End Page

23 / 25

Related Subject Headings

  • Biomedical Engineering
 

Citation

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MLA
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Iuliano, D. J., Proulx, T. L., Saavedra, S. S., & Truskey, G. A. (1991). Role of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, 21, 23–25.
Iuliano, D. J., T. L. Proulx, S. S. Saavedra, and G. A. Truskey. “Role of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow.” American Society of Mechanical Engineers, Bioengineering Division (Publication) BED 21 (December 1, 1991): 23–25.
Iuliano DJ, Proulx TL, Saavedra SS, Truskey GA. Role of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 1991 Dec 1;21:23–5.
Iuliano, D. J., et al. “Role of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow.” American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, vol. 21, Dec. 1991, pp. 23–25.
Iuliano DJ, Proulx TL, Saavedra SS, Truskey GA. Role of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 1991 Dec 1;21:23–25.

Published In

American Society of Mechanical Engineers, Bioengineering Division (Publication) BED

Publication Date

December 1, 1991

Volume

21

Start / End Page

23 / 25

Related Subject Headings

  • Biomedical Engineering