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Microstamping on an activated polymer surface: Patterning biotin and streptavidin onto common polymeric biomaterials

Publication ,  Journal Article
Hyun, J; Zhu, Y; Liebmann-Vinson, A; Beebe, TP; Chilkoti, A
Published in: Langmuir
October 2, 2001

Microstamping on an activated polymer surface (MAPS) is a methodology that enables biomolecules to be patterned on polymers with micrometer spatial resolution. MAPS combines homogeneous surface derivatization of a polymer to introduce a reactive functional group followed by reactive microcontact printing (μCP) of a biological ligand of interest, linked to an appropriate reactive group. We demonstrate here that polyethylene, polystyrene, poly(methyl methacrylate), and poly(ethylene terephthalate) films can be successfully modified to introduce COOH groups on their surfaces, which can be subsequently patterned by reactive μCP of amine-terminated biotin after derivatization of the COOH groups with pentafluorophenol. X-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry (TOF-SIMS) confirmed the chemistry of MAPS at each stage of the derivatization of the polymer surfaces and reactive μCP of biotin. Micropatterned biotin surfaces fabricated by MAPS were patterned with streptavidin by exploiting molecular recognition between biotin and streptavidin. The formation of streptavidin patterns was examined by fluorescence microscopy of Alexa488-labeled streptavidin and by TOF-SIMS imaging of 15N-labeled recombinant streptavidin, bound to biotin patterns. The contrast in the streptavidin micropatterns was optimized by examining the effect of blocking agents and streptavidin incubation time. Maximum contrast was obtained for binding of 0.1 μM streptavidin from a buffer containing 0.02% (v/v) Tween 20 detergent for an incubation time of 1 min.

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Published In

Langmuir

DOI

ISSN

0743-7463

Publication Date

October 2, 2001

Volume

17

Issue

20

Start / End Page

6358 / 6367

Related Subject Headings

  • Chemical Physics
 

Citation

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Hyun, J., Zhu, Y., Liebmann-Vinson, A., Beebe, T. P., & Chilkoti, A. (2001). Microstamping on an activated polymer surface: Patterning biotin and streptavidin onto common polymeric biomaterials. Langmuir, 17(20), 6358–6367. https://doi.org/10.1021/la010695x
Hyun, J., Y. Zhu, A. Liebmann-Vinson, T. P. Beebe, and A. Chilkoti. “Microstamping on an activated polymer surface: Patterning biotin and streptavidin onto common polymeric biomaterials.” Langmuir 17, no. 20 (October 2, 2001): 6358–67. https://doi.org/10.1021/la010695x.
Hyun J, Zhu Y, Liebmann-Vinson A, Beebe TP, Chilkoti A. Microstamping on an activated polymer surface: Patterning biotin and streptavidin onto common polymeric biomaterials. Langmuir. 2001 Oct 2;17(20):6358–67.
Hyun, J., et al. “Microstamping on an activated polymer surface: Patterning biotin and streptavidin onto common polymeric biomaterials.” Langmuir, vol. 17, no. 20, Oct. 2001, pp. 6358–67. Scopus, doi:10.1021/la010695x.
Hyun J, Zhu Y, Liebmann-Vinson A, Beebe TP, Chilkoti A. Microstamping on an activated polymer surface: Patterning biotin and streptavidin onto common polymeric biomaterials. Langmuir. 2001 Oct 2;17(20):6358–6367.
Journal cover image

Published In

Langmuir

DOI

ISSN

0743-7463

Publication Date

October 2, 2001

Volume

17

Issue

20

Start / End Page

6358 / 6367

Related Subject Headings

  • Chemical Physics