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Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization.

Publication ,  Journal Article
Schwartz, BL; Bruce, JE; Anderson, GA; Hofstadler, SA; Rockwood, AL; Smith, RD; Chilkoti, A; Stayton, PS
Published in: Journal of the American Society for Mass Spectrometry
June 1995

The noncovalent tetrameric association of the protein streptavidin formed by electrospray ionization (ESI) mass spectrometry has been observed intact and dissociated in the gas phase. An extended mass-to-charge ratio range quadrupole mass spectrometer was employed to examine the effects of harsher conditions in the ESI atmosphere-vacuum interface region on the streptavidin tetramer. Thermally induced dissociation caused the mass spectra to exhibit a series of complementary monomer and trimer ions that correspond to decomposition of the tetrameric species. Similar results were obtained with tandem mass spectrometric experiments on a Fourier transform ion cyclotron resonance mass spectrometer by application of sustained off-resonance irradiation (SORI) on a selected tetrameric charge state. The technique of single-frequency quadrupole excitation was used to accomplish selected-ion accumulation of the 14 + charge state of the tetramer during ion injection. Subsequent low energy SORI combined with broadband quadrupole cooling produced the 7 + monomer and 7 + trimer species, as well as the 6 + monomer and 8 + trimer complementary ions. The observed asymmetric breakup of the tetramer is qualitatively explained by using physical models.

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Published In

Journal of the American Society for Mass Spectrometry

DOI

EISSN

1879-1123

ISSN

1044-0305

Publication Date

June 1995

Volume

6

Issue

6

Start / End Page

459 / 465

Related Subject Headings

  • Analytical Chemistry
  • 3401 Analytical chemistry
  • 0306 Physical Chemistry (incl. Structural)
  • 0304 Medicinal and Biomolecular Chemistry
  • 0301 Analytical Chemistry
 

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Schwartz, B. L., Bruce, J. E., Anderson, G. A., Hofstadler, S. A., Rockwood, A. L., Smith, R. D., … Stayton, P. S. (1995). Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization. Journal of the American Society for Mass Spectrometry, 6(6), 459–465. https://doi.org/10.1016/1044-0305(95)00191-f
Schwartz, B. L., J. E. Bruce, G. A. Anderson, S. A. Hofstadler, A. L. Rockwood, R. D. Smith, A. Chilkoti, and P. S. Stayton. “Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization.Journal of the American Society for Mass Spectrometry 6, no. 6 (June 1995): 459–65. https://doi.org/10.1016/1044-0305(95)00191-f.
Schwartz BL, Bruce JE, Anderson GA, Hofstadler SA, Rockwood AL, Smith RD, et al. Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization. Journal of the American Society for Mass Spectrometry. 1995 Jun;6(6):459–65.
Schwartz, B. L., et al. “Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization.Journal of the American Society for Mass Spectrometry, vol. 6, no. 6, June 1995, pp. 459–65. Epmc, doi:10.1016/1044-0305(95)00191-f.
Schwartz BL, Bruce JE, Anderson GA, Hofstadler SA, Rockwood AL, Smith RD, Chilkoti A, Stayton PS. Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization. Journal of the American Society for Mass Spectrometry. 1995 Jun;6(6):459–465.
Journal cover image

Published In

Journal of the American Society for Mass Spectrometry

DOI

EISSN

1879-1123

ISSN

1044-0305

Publication Date

June 1995

Volume

6

Issue

6

Start / End Page

459 / 465

Related Subject Headings

  • Analytical Chemistry
  • 3401 Analytical chemistry
  • 0306 Physical Chemistry (incl. Structural)
  • 0304 Medicinal and Biomolecular Chemistry
  • 0301 Analytical Chemistry