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Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches".

Publication ,  Journal Article
Hyun, J; Lee, W-K; Nath, N; Chilkoti, A; Zauscher, S
Published in: Journal of the American Chemical Society
June 2004

This article describes the fabrication and characterization of stimulus-responsive elastin-like polypeptide (ELP) nanostructures grafted onto omega-substituted thiolates that were patterned onto gold surfaces by dip-pen nanolithography (DPN). In response to external stimuli such as changes in temperature or ionic strength, ELPs undergo a switchable and reversible, hydrophilic-hydrophobic phase transition at a lower critical solution temperature (LCST). We exploited this phase transition behavior to reversibly immobilize a thioredoxin-ELP (Trx-ELP) fusion protein onto the ELP nanopattern above the LCST. Subsequent binding of an anti-thioredoxin monoclonal antibody (anti-Trx) to the surface-captured thioredoxin showed the presentation of the immobilized protein in a sterically accessible orientation in the nanoarray. We also showed that the resulting Trx-ELP/anti-Trx complex formed above the LCST could be reversibly dissociated below the LCST. These results demonstrate the intriguing potential of ELP nanostructures as generic, reversible, biomolecular switches for on-chip capture and release of a small number (order 100-200) of protein molecules in integrated, nanoscale bioanalytical devices. We also investigated the molecular mechanism underlying this switch by measuring the height changes that accompany the binding and desorption steps and by adhesion force spectroscopy using atomic force microscopy.

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Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

June 2004

Volume

126

Issue

23

Start / End Page

7330 / 7335

Related Subject Headings

  • Tissue Adhesions
  • Sodium Chloride
  • Protein Binding
  • Protein Array Analysis
  • Peptides
  • Nanotechnology
  • Microscopy, Atomic Force
  • General Chemistry
  • Elastin
  • 40 Engineering
 

Citation

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MLA
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Hyun, J., Lee, W.-K., Nath, N., Chilkoti, A., & Zauscher, S. (2004). Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches". Journal of the American Chemical Society, 126(23), 7330–7335. https://doi.org/10.1021/ja049721e
Hyun, Jinho, Woo-Kyung Lee, Nidhi Nath, Ashutosh Chilkoti, and Stefan Zauscher. “Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches".Journal of the American Chemical Society 126, no. 23 (June 2004): 7330–35. https://doi.org/10.1021/ja049721e.
Hyun J, Lee W-K, Nath N, Chilkoti A, Zauscher S. Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches". Journal of the American Chemical Society. 2004 Jun;126(23):7330–5.
Hyun, Jinho, et al. “Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches".Journal of the American Chemical Society, vol. 126, no. 23, June 2004, pp. 7330–35. Epmc, doi:10.1021/ja049721e.
Hyun J, Lee W-K, Nath N, Chilkoti A, Zauscher S. Capture and release of proteins on the nanoscale by stimuli-responsive elastin-like polypeptide "switches". Journal of the American Chemical Society. 2004 Jun;126(23):7330–7335.
Journal cover image

Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

June 2004

Volume

126

Issue

23

Start / End Page

7330 / 7335

Related Subject Headings

  • Tissue Adhesions
  • Sodium Chloride
  • Protein Binding
  • Protein Array Analysis
  • Peptides
  • Nanotechnology
  • Microscopy, Atomic Force
  • General Chemistry
  • Elastin
  • 40 Engineering