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Molecular recognition-mediated fabrication of protein nanostructures by dip-pen lithography

Publication ,  Journal Article
Hyun, J; Ahn, SJ; Lee, WK; Chilkoti, A; Zauscher, S
Published in: Nano Lett. (USA)
2002

We describe the molecular recognition-mediated, stepwise fabrication of patterned protein nanostructures with feature sizes on the order of 200 nm. First, a self-assembled monolayer (SAM) of 16-mercaplohexadecanoic acid (MHA) is patterned onto gold by dip-pen nanolithography (DPN), and the unpatterned regions are passivated with a protein-resistant oligoethylene glycol-terminated alkanethiol SAM. Next, an amine-terminated biotin derivative is covalently conjugated with the chemically activated MHA SAM nanopattern. The surface is then incubated with streptavidin to form streptavidin nanostructures, mediated by molecular recognition between biotin and streptavidin. Finally, protein nanopatterns are fabricated by molecular recognition-mediated immobilization of biotinylated protein from solution. Our fabrication methodology is generically applicable because of the ubiquity of biotin-tagged molecules

Duke Scholars

Published In

Nano Lett. (USA)

DOI

Publication Date

2002

Volume

2

Issue

11

Start / End Page

1203 / 1207

Related Subject Headings

  • Nanoscience & Nanotechnology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hyun, J., Ahn, S. J., Lee, W. K., Chilkoti, A., & Zauscher, S. (2002). Molecular recognition-mediated fabrication of protein nanostructures by dip-pen lithography. Nano Lett. (USA), 2(11), 1203–1207. https://doi.org/10.1021/n10257364
Hyun, Jinho, Sang Jung Ahn, Woo Kyung Lee, A. Chilkoti, and S. Zauscher. “Molecular recognition-mediated fabrication of protein nanostructures by dip-pen lithography.” Nano Lett. (USA) 2, no. 11 (2002): 1203–7. https://doi.org/10.1021/n10257364.
Hyun J, Ahn SJ, Lee WK, Chilkoti A, Zauscher S. Molecular recognition-mediated fabrication of protein nanostructures by dip-pen lithography. Nano Lett (USA). 2002;2(11):1203–7.
Hyun, Jinho, et al. “Molecular recognition-mediated fabrication of protein nanostructures by dip-pen lithography.” Nano Lett. (USA), vol. 2, no. 11, 2002, pp. 1203–07. Manual, doi:10.1021/n10257364.
Hyun J, Ahn SJ, Lee WK, Chilkoti A, Zauscher S. Molecular recognition-mediated fabrication of protein nanostructures by dip-pen lithography. Nano Lett (USA). 2002;2(11):1203–1207.

Published In

Nano Lett. (USA)

DOI

Publication Date

2002

Volume

2

Issue

11

Start / End Page

1203 / 1207

Related Subject Headings

  • Nanoscience & Nanotechnology