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Control of protein-ligand recognition using a stimuli-responsive polymer.

Publication ,  Journal Article
Stayton, PS; Shimoboji, T; Long, C; Chilkoti, A; Chen, G; Harris, JM; Hoffman, AS
Published in: Nature
November 1995

Stimuli-responsive polymers exhibit reversible phase changes in response to changes in environmental factors such as pH or temperature. Conjugating such polymers to antibodies and proteins provides molecular systems for applications such as affinity separations, immunoassays and enzyme recovery and recycling. Here we show that conjugating a temperature-sensitive polymer to a genetically engineered site on a protein allows the protein's ligand binding affinity to be controlled. We synthesized a mutant of the protein streptavidin to enable site-specific conjugation of the responsive polymer near the protein's binding site. Normal binding of biotin to the modified protein occurs below 32 degrees C, whereas above this temperature the polymer collapses and blocks binding. The collapse of the polymer and thus the enabling and disabling of binding, is reversible. Such environmentally triggered control of binding may find many applications in biotechnology and biomedicine, such as the control of enzyme reaction rates and of biosensor activity, and the controlled release of drugs.

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Published In

Nature

DOI

EISSN

1476-4687

ISSN

0028-0836

Publication Date

November 1995

Volume

378

Issue

6556

Start / End Page

472 / 474

Related Subject Headings

  • Temperature
  • Sulfones
  • Streptavidin
  • Recombinant Proteins
  • Protein Conformation
  • Protein Binding
  • Polymers
  • Mutagenesis, Site-Directed
  • Models, Molecular
  • Ligands
 

Citation

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Stayton, P. S., Shimoboji, T., Long, C., Chilkoti, A., Chen, G., Harris, J. M., & Hoffman, A. S. (1995). Control of protein-ligand recognition using a stimuli-responsive polymer. Nature, 378(6556), 472–474. https://doi.org/10.1038/378472a0
Stayton, P. S., T. Shimoboji, C. Long, A. Chilkoti, G. Chen, J. M. Harris, and A. S. Hoffman. “Control of protein-ligand recognition using a stimuli-responsive polymer.Nature 378, no. 6556 (November 1995): 472–74. https://doi.org/10.1038/378472a0.
Stayton PS, Shimoboji T, Long C, Chilkoti A, Chen G, Harris JM, et al. Control of protein-ligand recognition using a stimuli-responsive polymer. Nature. 1995 Nov;378(6556):472–4.
Stayton, P. S., et al. “Control of protein-ligand recognition using a stimuli-responsive polymer.Nature, vol. 378, no. 6556, Nov. 1995, pp. 472–74. Epmc, doi:10.1038/378472a0.
Stayton PS, Shimoboji T, Long C, Chilkoti A, Chen G, Harris JM, Hoffman AS. Control of protein-ligand recognition using a stimuli-responsive polymer. Nature. 1995 Nov;378(6556):472–474.
Journal cover image

Published In

Nature

DOI

EISSN

1476-4687

ISSN

0028-0836

Publication Date

November 1995

Volume

378

Issue

6556

Start / End Page

472 / 474

Related Subject Headings

  • Temperature
  • Sulfones
  • Streptavidin
  • Recombinant Proteins
  • Protein Conformation
  • Protein Binding
  • Polymers
  • Mutagenesis, Site-Directed
  • Models, Molecular
  • Ligands