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An immobilized gold nanoparticle sensor for label free optical detection of biomolecular interactions

Publication ,  Journal Article
Nath, N; Chilkoti, A
Published in: Proceedings of SPIE - The International Society for Optical Engineering
January 1, 2002

We present a new label free optical technique to study biomolecular interactions in real time on a surface. This method monitors changes in the absorbance spectrum of a monolayer of gold on glass as a function of biomolecular binding. Gold nanoparticles with a diameter of 13 nm were chemisorbed onto an amine-terminated glass surface. The absorbance spectrum of the monolayer exhibited both a red shift as well as an increase in the absorbance at peak wavelength as a function of bulk solution refractive index. The increase in absorbance at 550 nm was employed to study the kinetics of fibrinogen adsorption on the immobilized monolayer. The results obtained with the absorbance sensor were compared with those obtained using conventional SPR. This sensor is attractive because of its simplicity: gold nanoparticles are easily prepared with high reproducibility, they can be easily immobilized on glass, and their absorbance spectrum can be easily measured using widely available UV-vis spectrophotometers. Furthermore, this technique should be easily amenable to the design of chips in an array format for application in high-throughput immunoassays and proteomics.

Duke Scholars

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

January 1, 2002

Volume

4626

Start / End Page

441 / 448

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering
 

Citation

APA
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ICMJE
MLA
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Nath, N., & Chilkoti, A. (2002). An immobilized gold nanoparticle sensor for label free optical detection of biomolecular interactions. Proceedings of SPIE - The International Society for Optical Engineering, 4626, 441–448. https://doi.org/10.1117/12.472110
Nath, N., and A. Chilkoti. “An immobilized gold nanoparticle sensor for label free optical detection of biomolecular interactions.” Proceedings of SPIE - The International Society for Optical Engineering 4626 (January 1, 2002): 441–48. https://doi.org/10.1117/12.472110.
Nath N, Chilkoti A. An immobilized gold nanoparticle sensor for label free optical detection of biomolecular interactions. Proceedings of SPIE - The International Society for Optical Engineering. 2002 Jan 1;4626:441–8.
Nath, N., and A. Chilkoti. “An immobilized gold nanoparticle sensor for label free optical detection of biomolecular interactions.” Proceedings of SPIE - The International Society for Optical Engineering, vol. 4626, Jan. 2002, pp. 441–48. Scopus, doi:10.1117/12.472110.
Nath N, Chilkoti A. An immobilized gold nanoparticle sensor for label free optical detection of biomolecular interactions. Proceedings of SPIE - The International Society for Optical Engineering. 2002 Jan 1;4626:441–448.

Published In

Proceedings of SPIE - The International Society for Optical Engineering

DOI

ISSN

0277-786X

Publication Date

January 1, 2002

Volume

4626

Start / End Page

441 / 448

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4009 Electronics, sensors and digital hardware
  • 4006 Communications engineering