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In vivo BOLD contrast MRI mapping of subcutaneous vascular function and maturation: validation by intravital microscopy.

Publication ,  Journal Article
Neeman, M; Dafni, H; Bukhari, O; Braun, RD; Dewhirst, MW
Published in: Magn Reson Med
May 2001

Bold contrast MRI was applied for mapping vascular maturation in tumor- and wound-induced skin angiogenesis using the response of mature vessels to hypercapnia (inhalation of air vs. air 5% CO(2)) and the response of all vessels to hyperoxia (air 5% CO(2) vs. oxygen 5% CO(2) (carbogen)). MRI signal enhancement with hypercapnia was reduced in centered vs. linear phase encoding, suggesting increased blood flow. However, intravital microscopy demonstrated constriction of arterioles and reduced flux and density of red blood cells in mature capillaries with hypercapnia, with no change in the diameter of wound-induced neovasculature. The discrepancy in flow between MRI and intravital microscopy is consistent with increased plasma flow and reduced hematocrit. Hyperoxia resulted in increased blood oxygenation and constriction of all vessels. These results provide a hemodynamic explanation for the selective registration of MRI response to hypercapnia with mature vessels and the response to hyperoxia with total vascular function.

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Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

May 2001

Volume

45

Issue

5

Start / End Page

887 / 898

Location

United States

Related Subject Headings

  • Wound Healing
  • Skin
  • Reproducibility of Results
  • Oxygen
  • Nuclear Medicine & Medical Imaging
  • Neovascularization, Pathologic
  • Mice, Nude
  • Mice
  • Magnetic Resonance Imaging
  • Hypercapnia
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Neeman, M., Dafni, H., Bukhari, O., Braun, R. D., & Dewhirst, M. W. (2001). In vivo BOLD contrast MRI mapping of subcutaneous vascular function and maturation: validation by intravital microscopy. Magn Reson Med, 45(5), 887–898. https://doi.org/10.1002/mrm.1118
Neeman, M., H. Dafni, O. Bukhari, R. D. Braun, and M. W. Dewhirst. “In vivo BOLD contrast MRI mapping of subcutaneous vascular function and maturation: validation by intravital microscopy.Magn Reson Med 45, no. 5 (May 2001): 887–98. https://doi.org/10.1002/mrm.1118.
Neeman M, Dafni H, Bukhari O, Braun RD, Dewhirst MW. In vivo BOLD contrast MRI mapping of subcutaneous vascular function and maturation: validation by intravital microscopy. Magn Reson Med. 2001 May;45(5):887–98.
Neeman, M., et al. “In vivo BOLD contrast MRI mapping of subcutaneous vascular function and maturation: validation by intravital microscopy.Magn Reson Med, vol. 45, no. 5, May 2001, pp. 887–98. Pubmed, doi:10.1002/mrm.1118.
Neeman M, Dafni H, Bukhari O, Braun RD, Dewhirst MW. In vivo BOLD contrast MRI mapping of subcutaneous vascular function and maturation: validation by intravital microscopy. Magn Reson Med. 2001 May;45(5):887–898.
Journal cover image

Published In

Magn Reson Med

DOI

ISSN

0740-3194

Publication Date

May 2001

Volume

45

Issue

5

Start / End Page

887 / 898

Location

United States

Related Subject Headings

  • Wound Healing
  • Skin
  • Reproducibility of Results
  • Oxygen
  • Nuclear Medicine & Medical Imaging
  • Neovascularization, Pathologic
  • Mice, Nude
  • Mice
  • Magnetic Resonance Imaging
  • Hypercapnia