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Human lung cancer cell lines exhibit resistance to retinoic acid treatment.

Publication ,  Journal Article
Geradts, J; Chen, JY; Russell, EK; Yankaskas, JR; Nieves, L; Minna, JD
Published in: Cell Growth Differ
October 1993

Retinoic acid (RA) and nuclear retinoic acid receptors (RARs) have been implicated in a variety of human malignancies including lung cancer, and RA has been proposed as a chemopreventive agent for bronchogenic carcinoma. Normal human tracheobronchial epithelial cells show dramatic induction of RAR-beta mRNA and significant growth inhibition after RA treatment. In contrast, 17 of 22 small cell lung cancer (SCLC) and 9 of 15 non-SCLC lines treated with 1 microM RA showed no significant growth inhibition. Of interest, 5 SCLC lines with high levels of myc gene family expression related to c-, N-, or L-myc gene amplification exhibited growth inhibition (28-87%), whereas 2 non-SCLC lines actually showed growth stimulation after treatment with 1 microM RA. The lines varied greatly in their constitutive expression of RAR-beta mRNA, and 15 of 20 SCLC and 8 of 15 non-SCLC lines failed to show RAR-beta mRNA induction after RA treatment. Six cell lines showed possible alterations in the coding region of RAR-beta by complementary DNA (cDNA)/polymerase chain reaction (PCR) analysis using primers common to the RAR-beta1,2,3 isoforms, since other regions would undergo cDNA/PCR amplification whereas the DNA binding domain would not. Nonetheless, no abnormal band shift patterns in cDNA amplified by PCR were found by single strand conformation polymorphism analysis covering all 1344 base pairs of the RAR-beta open reading frame. Finally, no abnormalities in RAR-alpha gene structure or expression were identified by Southern and Northern blot analysis, including lines with cytogenetic abnormalities of 17q21. We conclude that abnormalities of the RAR-beta system are common in human lung cancer cell lines.

Duke Scholars

Published In

Cell Growth Differ

ISSN

1044-9523

Publication Date

October 1993

Volume

4

Issue

10

Start / End Page

799 / 809

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Tretinoin
  • Receptors, Retinoic Acid
  • RNA, Messenger
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
  • Lung Neoplasms
  • Humans
  • Drug Resistance
 

Citation

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Geradts, J., Chen, J. Y., Russell, E. K., Yankaskas, J. R., Nieves, L., & Minna, J. D. (1993). Human lung cancer cell lines exhibit resistance to retinoic acid treatment. Cell Growth Differ, 4(10), 799–809.
Geradts, J., J. Y. Chen, E. K. Russell, J. R. Yankaskas, L. Nieves, and J. D. Minna. “Human lung cancer cell lines exhibit resistance to retinoic acid treatment.Cell Growth Differ 4, no. 10 (October 1993): 799–809.
Geradts J, Chen JY, Russell EK, Yankaskas JR, Nieves L, Minna JD. Human lung cancer cell lines exhibit resistance to retinoic acid treatment. Cell Growth Differ. 1993 Oct;4(10):799–809.
Geradts, J., et al. “Human lung cancer cell lines exhibit resistance to retinoic acid treatment.Cell Growth Differ, vol. 4, no. 10, Oct. 1993, pp. 799–809.
Geradts J, Chen JY, Russell EK, Yankaskas JR, Nieves L, Minna JD. Human lung cancer cell lines exhibit resistance to retinoic acid treatment. Cell Growth Differ. 1993 Oct;4(10):799–809.

Published In

Cell Growth Differ

ISSN

1044-9523

Publication Date

October 1993

Volume

4

Issue

10

Start / End Page

799 / 809

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Tretinoin
  • Receptors, Retinoic Acid
  • RNA, Messenger
  • Polymerase Chain Reaction
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
  • Lung Neoplasms
  • Humans
  • Drug Resistance