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Heterogeneity in the hormonal responsiveness of clones derived from the 13762NF rat mammary tumor.

Publication ,  Journal Article
Geradts, J; Richards, J; Edery, M; Pang, K; Larson, L; Nandi, S
Published in: Cancer Res
April 1986

The transplantable hormone-responsive rat mammary adenocarcinoma 13762NF was dissociated with collagenase and hyaluronidase. Cells were cloned directly or lines were established from mass cultures and cells from these lines were cloned. Clones differed in cellular morphology, colony morphology on plastic or in collagen gel, growth rate, growth response to hormones, and hormone receptor levels. Growth response to prolactin, estradiol, progesterone, cortisol, and epidermal growth factor (EGF) was determined by culturing the cells within collagen gel and using a serum-free medium base of DME/F12 (1:1) with insulin, linoleic acid, and BSA. The clones varied in their hormone responses, with all 20 of the clones tested responding to cortisol in combination with EGF. Some clones would respond to EGF, cortisol, or progesterone when used alone. None of the clones tested could be stimulated by prolactin or estradiol. Receptor levels for estradiol, progesterone, glucocorticoids, and EGF were assessed in 3 selected clones differing in their hormone responsiveness. Receptor levels appeared to correlate with hormonal sensitivity. Selected clones transplanted into female F344 rats produced carcinomas with histopathologies similar to the original tumor.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

April 1986

Volume

46

Issue

4 Pt 2

Start / End Page

1920 / 1927

Location

United States

Related Subject Headings

  • Receptors, Progesterone
  • Receptors, Glucocorticoid
  • Receptors, Cell Surface
  • Rats, Inbred F344
  • Rats
  • Prolactin
  • Progesterone
  • Oncology & Carcinogenesis
  • Neoplasms, Hormone-Dependent
  • Neoplasm Transplantation
 

Citation

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MLA
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Geradts, J., Richards, J., Edery, M., Pang, K., Larson, L., & Nandi, S. (1986). Heterogeneity in the hormonal responsiveness of clones derived from the 13762NF rat mammary tumor. Cancer Res, 46(4 Pt 2), 1920–1927.
Geradts, J., J. Richards, M. Edery, K. Pang, L. Larson, and S. Nandi. “Heterogeneity in the hormonal responsiveness of clones derived from the 13762NF rat mammary tumor.Cancer Res 46, no. 4 Pt 2 (April 1986): 1920–27.
Geradts J, Richards J, Edery M, Pang K, Larson L, Nandi S. Heterogeneity in the hormonal responsiveness of clones derived from the 13762NF rat mammary tumor. Cancer Res. 1986 Apr;46(4 Pt 2):1920–7.
Geradts, J., et al. “Heterogeneity in the hormonal responsiveness of clones derived from the 13762NF rat mammary tumor.Cancer Res, vol. 46, no. 4 Pt 2, Apr. 1986, pp. 1920–27.
Geradts J, Richards J, Edery M, Pang K, Larson L, Nandi S. Heterogeneity in the hormonal responsiveness of clones derived from the 13762NF rat mammary tumor. Cancer Res. 1986 Apr;46(4 Pt 2):1920–1927.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

April 1986

Volume

46

Issue

4 Pt 2

Start / End Page

1920 / 1927

Location

United States

Related Subject Headings

  • Receptors, Progesterone
  • Receptors, Glucocorticoid
  • Receptors, Cell Surface
  • Rats, Inbred F344
  • Rats
  • Prolactin
  • Progesterone
  • Oncology & Carcinogenesis
  • Neoplasms, Hormone-Dependent
  • Neoplasm Transplantation