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Suppressive effect on polyclonal B-cell activation of a synthetic peptide homologous to a transmembrane component of oncogenic retroviruses.

Publication ,  Journal Article
Mitani, M; Cianciolo, GJ; Snyderman, R; Yasuda, M; Good, RA; Day, NK
Published in: Proc Natl Acad Sci U S A
January 1987
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Purified feline leukemia virus, UV light-inactivated feline leukemia virus, and a synthetic peptide (CKS-17) homologous to a well-conserved region of the transmembrane components of several human and animal retroviruses were each studied for their effects on IgG production by feline peripheral blood lymphocytes. Using a reverse hemolytic plaque assay, both the viable virus and the UV-inactivated feline leukemia virus, but not the CKS-17, activated B lymphocytes to secrete IgG. When staphylococcal protein A, a polyclonal B-cell activator, was used to stimulate IgG synthesis by feline lymphocytes, the viable virus, the UV-inactivated virus, and the CKS-17 peptide each strongly suppressed IgG secretion without compromising viability of the lymphocytes. These findings suggest that the immunosuppressive influences of feline leukemia virus on immunoglobulin synthesis may reside in a conserved portion of the envelope glycoprotein that includes the region homologous to CKS-17.

Duke Scholars

George James Cianciolo
Author George James Cianciolo Pathology
Ralph Snyderman
Author Ralph Snyderman Medicine
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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.84.1.237

ISSN

0027-8424

Publication Date

January 1987

Volume

84

Issue

1

Start / End Page

237 / 240

Location

United States

Related Subject Headings

  • Viral Proteins
  • Ultraviolet Rays
  • Peptides
  • Male
  • Lymphocyte Activation
  • Leukemia Virus, Feline
  • Immunosuppression Therapy
  • Immunoglobulin G
  • Female
  • Cats
 

Citation

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Mitani, M., Cianciolo, G. J., Snyderman, R., Yasuda, M., Good, R. A., & Day, N. K. (1987). Suppressive effect on polyclonal B-cell activation of a synthetic peptide homologous to a transmembrane component of oncogenic retroviruses. Proc Natl Acad Sci U S A, 84(1), 237–240. https://doi.org/10.1073/pnas.84.1.237
Mitani, M., G. J. Cianciolo, R. Snyderman, M. Yasuda, R. A. Good, and N. K. Day. “Suppressive effect on polyclonal B-cell activation of a synthetic peptide homologous to a transmembrane component of oncogenic retroviruses.” Proc Natl Acad Sci U S A 84, no. 1 (January 1987): 237–40. https://doi.org/10.1073/pnas.84.1.237.
Mitani M, Cianciolo GJ, Snyderman R, Yasuda M, Good RA, Day NK. Suppressive effect on polyclonal B-cell activation of a synthetic peptide homologous to a transmembrane component of oncogenic retroviruses. Proc Natl Acad Sci U S A. 1987 Jan;84(1):237–40.
Mitani, M., et al. “Suppressive effect on polyclonal B-cell activation of a synthetic peptide homologous to a transmembrane component of oncogenic retroviruses.” Proc Natl Acad Sci U S A, vol. 84, no. 1, Jan. 1987, pp. 237–40. Pubmed, doi:10.1073/pnas.84.1.237.
Mitani M, Cianciolo GJ, Snyderman R, Yasuda M, Good RA, Day NK. Suppressive effect on polyclonal B-cell activation of a synthetic peptide homologous to a transmembrane component of oncogenic retroviruses. Proc Natl Acad Sci U S A. 1987 Jan;84(1):237–240.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.84.1.237

ISSN

0027-8424

Publication Date

January 1987

Volume

84

Issue

1

Start / End Page

237 / 240

Location

United States

Related Subject Headings

  • Viral Proteins
  • Ultraviolet Rays
  • Peptides
  • Male
  • Lymphocyte Activation
  • Leukemia Virus, Feline
  • Immunosuppression Therapy
  • Immunoglobulin G
  • Female
  • Cats