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Inhibition of murine cytotoxic T lymphocyte activity by a synthetic retroviral peptide and abrogation of this activity by IL.

Publication ,  Journal Article
Ogasawara, M; Haraguchi, S; Cianciolo, GJ; Mitani, M; Good, RA; Day, NK
Published in: J Immunol
July 15, 1990

A synthetic 17 amino acid peptide (CKS-17) homologous to a highly conserved region of human and animal retroviral transmembrane proteins was investigated for its influence on the generation of murine alloantigen-specific CTL activity in vitro. CKS-17 coupled to a carrier protein, BSA or human serum albumin, inhibited the generation of anti-allo CTL in a dose-dependent manner. Controls consisting of BSA and human serum albumin, which had undergone the coupling procedure or neurotensin, an unrelated peptide, coupled to BSA in an identical manner as CKS-17 showed no such inhibitory action. The suppression was not restricted to the Ag specificity of the CTL activity. CKS-17 exerted inhibitory effects on the early afferent phase of CTL induction. Kinetic studies showed that the suppressive activity occurred when CKS-17 was introduced to the immunologically stimulating culture concomitant with or up to 48 h after initiation of culture. Analysis of the frequency of CTL precursor cells using limiting-dilution assays revealed that CKS-17 did act to reduce the number of precursor cells. Abrogation of the inhibition of CTL activity was observed when IL-2 was introduced to the culture together with the stimulator cells. Other lymphokines, such as IL-4, exerted a similar influence to counteract this suppression.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1990

Volume

145

Issue

2

Start / End Page

456 / 462

Location

United States

Related Subject Headings

  • Viral Envelope Proteins
  • Time Factors
  • T-Lymphocytes, Cytotoxic
  • Retroviridae Proteins
  • Peptides
  • Mice, Inbred Strains
  • Mice
  • Interleukin-4
  • Interleukin-2
  • Intercellular Signaling Peptides and Proteins
 

Citation

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MLA
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Ogasawara, M., Haraguchi, S., Cianciolo, G. J., Mitani, M., Good, R. A., & Day, N. K. (1990). Inhibition of murine cytotoxic T lymphocyte activity by a synthetic retroviral peptide and abrogation of this activity by IL. J Immunol, 145(2), 456–462.
Ogasawara, M., S. Haraguchi, G. J. Cianciolo, M. Mitani, R. A. Good, and N. K. Day. “Inhibition of murine cytotoxic T lymphocyte activity by a synthetic retroviral peptide and abrogation of this activity by IL.J Immunol 145, no. 2 (July 15, 1990): 456–62.
Ogasawara M, Haraguchi S, Cianciolo GJ, Mitani M, Good RA, Day NK. Inhibition of murine cytotoxic T lymphocyte activity by a synthetic retroviral peptide and abrogation of this activity by IL. J Immunol. 1990 Jul 15;145(2):456–62.
Ogasawara M, Haraguchi S, Cianciolo GJ, Mitani M, Good RA, Day NK. Inhibition of murine cytotoxic T lymphocyte activity by a synthetic retroviral peptide and abrogation of this activity by IL. J Immunol. 1990 Jul 15;145(2):456–462.

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1990

Volume

145

Issue

2

Start / End Page

456 / 462

Location

United States

Related Subject Headings

  • Viral Envelope Proteins
  • Time Factors
  • T-Lymphocytes, Cytotoxic
  • Retroviridae Proteins
  • Peptides
  • Mice, Inbred Strains
  • Mice
  • Interleukin-4
  • Interleukin-2
  • Intercellular Signaling Peptides and Proteins