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Production of a retroviral P15E-related chemotaxis inhibitor by IL-1-treated endothelial cells. A possible negative feedback in the regulation of the vascular response to monokines.

Publication ,  Journal Article
Wang, JM; Chen, ZG; Cianciolo, GJ; Snyderman, R; Breviario, F; Dejana, E; Mantovani, A
Published in: J Immunol
March 15, 1989

The effects of IL-1 on vascular endothelium result in a complex set of alterations which are potentially disruptive of vessel wall and underlying tissue integrity. The present study was aimed at investigating possible regulation of such potentially destructive responses elicited by IL-1 on endothelial cells. Culture supernatants of IL-1-treated human umbilical vein endothelial cells (HEC) were depleted of retroviral p15E-related Ag with immobilized anti-p15E mAb. The monocyte chemotactic and polarizing activity of supernatants of IL-1-treated HEC (presumably related to colony-stimulating factors being released by HEC) was markedly augmented by absorption on immobilized anti-p15E antibodies. Irrelevant IgG had no effect and anti-p15E antibodies did not affect the chemotactic activity of supernatants from unstimulated HEC. The material eluted from Sepharose-bound anti-p15E antibodies was devoid of chemotactic and polarizing activity and suppressed the polarization and migration of monocytes in response to chemoattractants. The alpha and beta molecular species of IL-1 were equally effective in inducing the production of p15E-related inhibitor. The production of a p15E-related inhibitor of chemotaxis induced by IL-1 in HEC may represent a negative signal in the regulation of the potentially destructive responses to pro-inflammatory cytokines.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

March 15, 1989

Volume

142

Issue

6

Start / End Page

2012 / 2017

Location

United States

Related Subject Headings

  • Retroviridae Proteins, Oncogenic
  • Retroviridae Proteins
  • Recombinant Proteins
  • Monocytes
  • Mice
  • Leukemia Virus, Murine
  • Interleukin-1
  • Immunology
  • Humans
  • Gene Products, gag
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, J. M., Chen, Z. G., Cianciolo, G. J., Snyderman, R., Breviario, F., Dejana, E., & Mantovani, A. (1989). Production of a retroviral P15E-related chemotaxis inhibitor by IL-1-treated endothelial cells. A possible negative feedback in the regulation of the vascular response to monokines. J Immunol, 142(6), 2012–2017.
Wang, J. M., Z. G. Chen, G. J. Cianciolo, R. Snyderman, F. Breviario, E. Dejana, and A. Mantovani. “Production of a retroviral P15E-related chemotaxis inhibitor by IL-1-treated endothelial cells. A possible negative feedback in the regulation of the vascular response to monokines.J Immunol 142, no. 6 (March 15, 1989): 2012–17.
Wang JM, Chen ZG, Cianciolo GJ, Snyderman R, Breviario F, Dejana E, Mantovani A. Production of a retroviral P15E-related chemotaxis inhibitor by IL-1-treated endothelial cells. A possible negative feedback in the regulation of the vascular response to monokines. J Immunol. 1989 Mar 15;142(6):2012–2017.

Published In

J Immunol

ISSN

0022-1767

Publication Date

March 15, 1989

Volume

142

Issue

6

Start / End Page

2012 / 2017

Location

United States

Related Subject Headings

  • Retroviridae Proteins, Oncogenic
  • Retroviridae Proteins
  • Recombinant Proteins
  • Monocytes
  • Mice
  • Leukemia Virus, Murine
  • Interleukin-1
  • Immunology
  • Humans
  • Gene Products, gag