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Human IFN-gamma production is inhibited by a synthetic peptide homologous to retroviral envelope protein.

Publication ,  Journal Article
Ogasawara, M; Cianciolo, GJ; Snyderman, R; Mitani, M; Good, RA; Day, NK
Published in: J Immunol
July 15, 1988

A synthetic 17 amino acid peptide (CKS-17) homologous to a highly conserved region of human and animal retroviral transmembrane proteins was investigated for its influence on the in vitro production of IFN-gamma from human peripheral mononuclear cells. The results showed that CKS-17 coupled to a carrier protein, BSA, inhibited production of IFN-gamma in a dose-dependent manner. Controls, consisting of BSA, which had undergone the coupling procedure or neurotensin coupled to BSA in an identical manner as CKS-17, showed no such inhibition. Reduction in IFN-gamma production could not be attributed to decreased viability of cells, delay of IFN-gamma production or to involvement of suppressor cells. Moreover, inhibition of IFN-gamma production was not related to the inhibition of DNA synthesis. The inhibition appeared to be a direct effect of CKS-17 on IFN-gamma-producing cells. Kinetic studies revealed that this suppression occurred when CKS-17 was introduced to the culture concurrent with or within 48 h after introduction of IFN inducers. Preincubation experiments showed that the presence of CKS-17 in the culture medium was not necessary to exert its inhibitory effect. These results suggest that a portion of retroviral envelope proteins possess important immunomodulatory actions.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1988

Volume

141

Issue

2

Start / End Page

614 / 619

Location

United States

Related Subject Headings

  • Viral Envelope Proteins
  • Thymidine
  • T-Lymphocytes, Regulatory
  • Staphylococcus aureus
  • Serum Albumin, Bovine
  • Sequence Homology, Nucleic Acid
  • Retroviridae Proteins
  • Protein Synthesis Inhibitors
  • Peptides
  • Molecular Sequence Data
 

Citation

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Ogasawara, M., Cianciolo, G. J., Snyderman, R., Mitani, M., Good, R. A., & Day, N. K. (1988). Human IFN-gamma production is inhibited by a synthetic peptide homologous to retroviral envelope protein. J Immunol, 141(2), 614–619.
Ogasawara, M., G. J. Cianciolo, R. Snyderman, M. Mitani, R. A. Good, and N. K. Day. “Human IFN-gamma production is inhibited by a synthetic peptide homologous to retroviral envelope protein.J Immunol 141, no. 2 (July 15, 1988): 614–19.
Ogasawara M, Cianciolo GJ, Snyderman R, Mitani M, Good RA, Day NK. Human IFN-gamma production is inhibited by a synthetic peptide homologous to retroviral envelope protein. J Immunol. 1988 Jul 15;141(2):614–9.
Ogasawara, M., et al. “Human IFN-gamma production is inhibited by a synthetic peptide homologous to retroviral envelope protein.J Immunol, vol. 141, no. 2, July 1988, pp. 614–19.
Ogasawara M, Cianciolo GJ, Snyderman R, Mitani M, Good RA, Day NK. Human IFN-gamma production is inhibited by a synthetic peptide homologous to retroviral envelope protein. J Immunol. 1988 Jul 15;141(2):614–619.

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 15, 1988

Volume

141

Issue

2

Start / End Page

614 / 619

Location

United States

Related Subject Headings

  • Viral Envelope Proteins
  • Thymidine
  • T-Lymphocytes, Regulatory
  • Staphylococcus aureus
  • Serum Albumin, Bovine
  • Sequence Homology, Nucleic Acid
  • Retroviridae Proteins
  • Protein Synthesis Inhibitors
  • Peptides
  • Molecular Sequence Data