Contribution of the VH11 gene family to mitogen-responsive B cell repertoire in C57BL/6 mice.
The contribution of VH11 gene family to the development of the primary B cell repertoire has been studied by analyzing 1.8 x 10(4) mitogen induced B lymphocyte colonies. The data demonstrate that VH11 family is predominantly expressed among neonatal splenic as well as adult peritoneal B cell colonies, both rich in Ly-1+ B cells. VH11 gene family expression among B splenocytes decreases during ontogeny and VH11 family pairs stochastically with different V kappa families among mitogen-activated neonatal B cell colonies, which are representative of an antigen unselected B cell repertoire. Thus, an increased VH11 expression among peritoneal and neonatal B cells points towards its biased expression among Ly-1+ B lymphocytes. The restricted V gene rearrangements and VH11-V kappa 9 pairing observed among anti-bromelain-treated mouse red blood cells autoantibodies are likely to be an outcome of both intrinsic gene recombination processes per se as well as selection by an autoantigen and/or local selective environmental factors.
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Related Subject Headings
- Nucleic Acid Hybridization
- Mitogens
- Mice, Inbred C57BL
- Mice
- Immunology
- Immunoglobulin Variable Region
- Immunoglobulin Light Chains
- Immunoglobulin Heavy Chains
- Genes, Immunoglobulin
- Gene Expression
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Nucleic Acid Hybridization
- Mitogens
- Mice, Inbred C57BL
- Mice
- Immunology
- Immunoglobulin Variable Region
- Immunoglobulin Light Chains
- Immunoglobulin Heavy Chains
- Genes, Immunoglobulin
- Gene Expression