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RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues.

Publication ,  Journal Article
Monroe, RJ; Seidl, KJ; Gaertner, F; Han, S; Chen, F; Sekiguchi, J; Wang, J; Ferrini, R; Davidson, L; Kelsoe, G; Alt, FW
Published in: Immunity
August 1999

We generated mice in which a functional RAG2:GFP fusion gene is knocked in to the endogenous RAG2 locus. In bone marrow and thymus, RAG2:GFP expression occurs in appropriate stages of developing B and T cells as well as in immature bone marrow IgM+ B cells. RAG2:GFP also is expressed in IgD+ B cells following cross-linking of IgM on immature IgM+ IgD+ B cells generated in vitro. RAG2:GFP expression is undetectable in most immature splenic B cells; however, in young RAG2:GFP mice, there are substantial numbers of splenic RAG2:GFP+ cells that mostly resemble pre-B cells. The latter population decreases in size with age but reappears following immunization of older RAG2:GFP mice. We discuss the implications of these findings for current models of receptor assembly and diversification.

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Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

August 1999

Volume

11

Issue

2

Start / End Page

201 / 212

Location

United States

Related Subject Headings

  • Recombination, Genetic
  • Recombinant Fusion Proteins
  • Receptors, Interleukin-2
  • Receptors, Antigen, B-Cell
  • Mice
  • Lymphoid Tissue
  • Lymphocytes
  • Luminescent Proteins
  • Immunology
  • Immunoglobulin D
 

Citation

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Monroe, R. J., Seidl, K. J., Gaertner, F., Han, S., Chen, F., Sekiguchi, J., … Alt, F. W. (1999). RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues. Immunity, 11(2), 201–212. https://doi.org/10.1016/s1074-7613(00)80095-3
Monroe, R. J., K. J. Seidl, F. Gaertner, S. Han, F. Chen, J. Sekiguchi, J. Wang, et al. “RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues.Immunity 11, no. 2 (August 1999): 201–12. https://doi.org/10.1016/s1074-7613(00)80095-3.
Monroe RJ, Seidl KJ, Gaertner F, Han S, Chen F, Sekiguchi J, et al. RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues. Immunity. 1999 Aug;11(2):201–12.
Monroe, R. J., et al. “RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues.Immunity, vol. 11, no. 2, Aug. 1999, pp. 201–12. Pubmed, doi:10.1016/s1074-7613(00)80095-3.
Monroe RJ, Seidl KJ, Gaertner F, Han S, Chen F, Sekiguchi J, Wang J, Ferrini R, Davidson L, Kelsoe G, Alt FW. RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues. Immunity. 1999 Aug;11(2):201–212.
Journal cover image

Published In

Immunity

DOI

ISSN

1074-7613

Publication Date

August 1999

Volume

11

Issue

2

Start / End Page

201 / 212

Location

United States

Related Subject Headings

  • Recombination, Genetic
  • Recombinant Fusion Proteins
  • Receptors, Interleukin-2
  • Receptors, Antigen, B-Cell
  • Mice
  • Lymphoid Tissue
  • Lymphocytes
  • Luminescent Proteins
  • Immunology
  • Immunoglobulin D